论文部分内容阅读
目的研究孕妇外周血游离胎儿DNA(fDNA)是否与子前期及其严重程度相关,检测子前期孕妇体内抗单链DNA抗体(抗ssDNA抗体)及抗双链DNA抗体(抗dsDNA抗体)是否存在异常变化。方法选择2005年5月至2006年11月在浙江省人民医院妇产科住院的20例轻度子前期孕妇(轻度子前期组),14例重度子前期孕妇(重度子前期组),42例无妊娠合并症及并发症的孕妇(对照组),所有孕妇均妊娠男性胎儿。抽取孕妇外周血后采用实时荧光定量PCR技术(FQ-PCR)对fDNA进行定量,同时采用ELISA技术对抗ssDNA抗体和抗dsDNA抗体定量。结果对照组、轻度子前期组和重度子前期组的fDNA均值分别为(67.83±43.70)拷贝/mL、(172.31±127.89)拷贝/mL、(321.37±47.07)拷贝/mL,差异有统计学意义(χ2=21.8514,P<0.01);三组的抗ssD-NA抗体光密度均值(OD)分别为(0.067±0.074)、(0.075±0.039)、(0.160±0.214),差异有统计学意义(χ2=6.3741,P<0.05);而不同组间抗dsDNA抗体OD均值为(0.0230±0.0102)、(0.0257±0.0099)、(0.0281±0.0119),差异无统计学意义(χ2=2.6340,P>0.05);抗ssDNA抗体与抗dsDNA抗体之间存在显著的相关性(r=0.5985,P<0.05),抗ssDNA抗体、抗dsDNA抗体及新生儿体重与fDNA浓度均无显著的相关性(P>0.05)。结论fDNA与子前期的严重程度密切相关,抗DNA抗体在子前期的发病过程中可能起着重要的作用。
Objective To study whether fetus peripheral blood free fetal DNA (fDNA) is correlated with pre-natal and its severity, to detect whether anti-single-stranded DNA (anti-ssDNA) and anti-dsDNA There are abnormal changes. Methods From May 2005 to November 2006 in Zhejiang Provincial People ’s Hospital Obstetrics and Gynecology hospitalized 20 cases of mild preeclampsia pregnant women (mild preeclampsia group), 14 cases of severe preeclampsia pregnant women (severe preeclampsia Group), 42 pregnant women without pregnancy complications and complications (control group), all pregnant women were pregnant male fetuses. FQ-PCR was used to quantify fDNA after pregnant women’s peripheral blood was extracted, and ELISA and anti-dsDNA antibodies were used to quantify them. Results The fDNA mean values of the control group, pre-mild preeclampsia group and pre-severe preeclampsia group were (67.83 ± 43.70) copies / mL, (172.31 ± 127.89) copies / mL and (321.37 ± 47.07) copies / mL, respectively (Χ2 = 21.8514, P <0.01). The optical density OD values of the three groups were (0.067 ± 0.074), (0.075 ± 0.039) and (0.160 ± 0.214), respectively (Χ2 = 6.3741, P <0.05). However, the OD value of anti-dsDNA antibody in different groups was (0.0230 ± 0.0102), (0.0257 ± 0.0099) and (0.0281 ± 0.0119) respectively, with no significant difference (χ2 = There was a significant correlation between anti-ssDNA antibody and anti-dsDNA antibody (r = 0.5985, P <0.05). There was no significant correlation between anti-ssDNA antibody, anti-dsDNA antibody and neonatal body weight and fDNA concentration P> 0.05). Conclusion The fDNA is closely related to the severity of preeclampsia and anti-DNA antibody may play an important role in the pathogenesis of preeclampsia.