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目的 :探寻羟乙基淀粉治疗大鼠蛋白尿的可行性。方法 :建立蛋白尿大鼠动物模型 ,在静脉点滴 6 %羟乙基淀粉前后 ,通过大鼠输尿管插管收集肾后膀胱前尿液 ,测定尿蛋白分子量并比较治疗前后尿蛋白含量 ,采用聚丙烯酰胺凝胶电泳法对尿蛋白分子量进行分析 ,同时观察治疗前后血肌酐和尿素氮变化。结果 :羟乙基淀粉治疗后尿蛋白定量较治疗前明显下降 ,尿蛋白电泳表明高、中分子蛋白条带数目和含量较治疗前显著减少。治疗前后尿量、血肌酐和尿素氮无明显变化。结论 :羟乙基淀粉能够安全、有效地减少实验大鼠的蛋白尿 ,为探寻蛋白尿的新的治疗方法提供了理论依据
Objective: To explore the feasibility of hydroxyethyl starch in the treatment of proteinuria in rats. Methods: The animal model of albuminuria was established. Before and after intravenous infusion of 6% hydroxyethyl starch, the urinary bladder was collected by ureteral intubation in rats. The urinary protein molecular weight was measured and compared before and after treatment. Polypropylene Amylolytic gel electrophoresis was used to analyze the molecular weight of urine protein and to observe the changes of serum creatinine and urea nitrogen before and after treatment. Results: After the treatment of hydroxyethyl starch, the urinary protein quantitation was significantly lower than that before treatment, and the urinary protein electrophoresis showed high and the number and content of the middle molecular protein bands were significantly reduced than before treatment. Before and after treatment, urine output, serum creatinine and urea nitrogen did not change significantly. Conclusion: Hydroxyethyl starch can reduce the proteinuria of experimental rats safely and effectively, providing a theoretical basis for exploring new therapeutic methods of proteinuria