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目的:研究8-甲氧补骨脂素(8-MOP)对可卡因(Coc)致小鼠急性肝损伤的保护作用。方法:采用Coc所致小鼠急性肝损伤模型。动物随机分为6组:正常对照组,8-MOP对照组,模型组,8-MOP低、中、高剂量组。正常对照组和模型组给予蒸馏水,8-MOP对照组、8-MOP组给予8-MOP,均为灌胃给药,每天1次,连续4d;最后1次给药30min后,除正常对照组和8-MOP对照组外,其余各组均皮下注射Coc制备小鼠急性肝损伤模型。24h后,检测小鼠血清中丙氨酸氨基转移酶(ALT);留取肝组织,病理染色,光镜观察肝组织病理变化;制备肝匀浆,测定肝组织中还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)和丙二醛(MDA)的含量。结果:与正常对照组比较,模型组小鼠血清中ALT活性明显升高,肝组织出现明显的肝细胞变性坏死;与模型组相比,8-MOP可以明显降低小鼠血清中ALT的活性,降低肝组织中MDA的含量,升高GSH/GSSG比值,肝组织病理损伤也明显减轻。结论:8-MOP对Coc致小鼠急性肝损伤具有明显的保护作用。
Objective: To study the protective effect of 8-MOP on acute liver injury induced by cocaine in mice. Methods: Acute liver injury induced by Coc was induced in mice. Animals were randomly divided into 6 groups: normal control group, 8-MOP control group, model group, 8-MOP low, medium and high dose groups. Normal control group and model group given distilled water, 8-MOP control group, 8-MOP group were given 8-MOP, were intragastric administration, once daily for 4d; the last 30min after administration, except for the normal control group And 8-MOP control group, the other groups were subcutaneously injected with Coc mouse acute liver injury model. Twenty-four hours later, the alanine aminotransferase (ALT) was detected in the serum of the mice; the liver tissues were collected for histopathological staining and histopathological changes were observed under light microscope; liver homogenates were prepared for determination of reduced glutathione GSH), oxidized glutathione (GSSG) and malondialdehyde (MDA) content. Results: Compared with the normal control group, the ALT activity in the serum of the model group was significantly increased, and hepatic degeneration and necrosis of the liver tissue was obvious. Compared with the model group, 8-MOP could significantly reduce the activity of ALT in the mouse serum, Reduce the content of MDA in liver tissue and increase the ratio of GSH / GSSG, the pathological damage of liver tissue was also significantly reduced. Conclusion: 8-MOP has a significant protective effect on Coc-induced acute liver injury in mice.