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目的探讨新生儿胎粪中肠三叶因子(ITF)的影响因素。方法收集我院出生新生儿的胎粪和血清标本,根据胎龄和Apgar评分将研究对象分为正常足月对照组、早产儿组和窒息组。采用高效液相色谱法测定胎粪中ITF的含量,采用ELISA法测定血清中胃动素(MOT)、胃泌素(GAS)和生长抑素(SS)。结果①胎粪中ITF的含量与胎龄的相关系数r为0.526,P为0.002。②早产儿组和窒息儿组胎粪中ITF含量均明显低于对照组,差别有统计学意义。③胎粪中ITF含量与血清中MOT、GAS及SS浓度的相关系数分别为-0.692,-0.361,0.213,P值分别为0.042,0.150,0.251。④对照组胎粪中ITF含量男性为(3.793±0.381)ng/g,女性为(4.108±0.429)ng/g,差异无统计学意义(P=0.129)。⑤所有研究对象中无1例坏死性小肠结肠炎(NEC)发生,但早产儿组有4例发生明显的喂养不耐受;窒息组有2例出现轻微的喂养不耐受。结论早产、窒息儿粪便中的ITF含量低于正常足月儿,推测肠道ITF分泌不足可能是导致早产儿和窒息儿容易发生肠道粘膜损害的原因之一。但进一步的系统研究非常必要。
Objective To investigate the influencing factors of midgut midgut trefoil factor (ITF) in neonates. Methods Meconium and serum samples of neonates born in our hospital were collected. Subjects were divided into normal term control group, premature infant group and asphyxia group according to gestational age and Apgar score. The contents of ITF in meconium were determined by high performance liquid chromatography (HPLC) and motilin, gastrin (GAS) and somatostatin (SS) were measured by ELISA. Results ① The correlation coefficient of ITF content in gestational pigs with gestational age was 0.526 and P was 0.002. ② The content of ITF in preterm infants and asphyxiated infants was significantly lower than that in the control group, the difference was statistically significant. ③ The correlation coefficient of ITF content in serum and MOT, GAS and SS in serum were -0.692, -0.361 and 0.213 respectively, P values were 0.042, 0.150 and 0.251 respectively. ④ In control group, the ITF content of meconium was (3.793 ± 0.381) ng / g in men and (4.108 ± 0.429) ng / g in female, respectively, with no significant difference (P = 0.129). (5) Necrotic enterocolitis (NEC) occurred in none of the subjects, but significant feeding intolerance occurred in 4 of the preterm infants and mild feeding intolerance in 2 of the asphyxiated patients. Conclusions The ITF content in stool of premature and asphyxiated children is lower than that of normal full-term children. It is speculated that inadequate secretion of intestinal ITF may be one of the causes of intestinal mucosal damage in preterm and asphyxiated children. However, further systematic research is necessary.