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目的观察南药山稔子、银杏、灵芝及火麻仁组合物对体外和体内DNA氧化损伤的保护作用。方法原代培养脾淋巴细胞,以H2O2为氧化受试物,比较空白对照组、阳性对照组和不同浓度南药组合物保护组彗星细胞率和DNA迁移长度。建立小鼠氧化应激组和南药保护组,分别测定血清总抗氧化能力(TAP)等指标以及血清中8-OHdG的浓度。结果南药组合物能不同程度地降低H2O2诱导产生的DNA损伤,以稀释20倍保护效果最好。用药后模型小鼠血清TAP[(19.63±1.59)U/ml]、肝脏组织丙二醛[MDA,(3.62±0.28)nmol/10mgprotein]和血清8-OHdG[(31.45±2.31)ng/ml]水平高于模型组;南药保护组TAP、MDA以及血清8-OHdG的浓度与氧化应激组比较,差别有统计学意义(P<0.01)。结论该南药组合物具有抗氧化作用,能在一定浓度范围内显著减少氧自由基对体内、体外DNA的氧化损伤。
Objective To observe the protective effects of the composition of Radix Astragali, Ginkgo biloba, Ganoderma lucidum and Hempen on DNA oxidation damage in vitro and in vivo. Methods Primary spleen lymphocytes were cultured with H2O2 as the oxidized test substance. The rate of comet cells and the length of DNA migration were compared between blank control group, positive control group and different concentrations of the South drug composition protection group. Mouse oxidative stress group and southern medicine protection group were established. Serum total antioxidative capacity (TAP) and other indicators as well as serum 8-OHdG concentrations were determined. Results The Southern drug composition can reduce the DNA damage induced by H2O2 in different degrees, and the protection effect is best diluted 20 times. After treatment, serum TAP [(19.63±1.59) U/ml], liver tissue malondialdehyde (MDA, (3.62±0.28) nmol/10 mg protein) and serum 8-OHdG [(31.45±2.31) ng/ml] The levels of TAP, MDA and serum 8-OHdG in the South China Medical Protection group were significantly higher than those in the oxidative stress group (P<0.01). Conclusion The South medicine composition has anti-oxidation effect and can significantly reduce the oxidative damage of oxygen free radicals to DNA in vitro and in vivo in a certain concentration range.