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目的探讨基因组总体甲基化水平与儿童原发性肾病综合征的关系。方法收集本院儿童肾内科诊断为原发性肾病综合征患儿20例为肾病组,另收集本院儿童保健科体检儿童20例为健康对照组,采用ELISA法检测肾病患儿缓解前后的总体甲基化水平变化。结果肾病组的总体甲基化水平为(0.343±0.054)%,健康对照组的总体甲基化水平为(0.870±0.116)%,2组差异有统计学意义(P<0.01)。同时检测18例激素敏感型肾病综合征患儿肾病缓解前后的总体甲基化水平,肾病缓解前总体甲基化水平为(0.340±0.058)%,肾病缓解后总体甲基化水平为(0.395±0.049)%,2组差异无统计学意义(P>0.05)。结论原发性肾病综合征患儿的总体甲基化水平降低,激素敏感型肾病综合征患儿肾病缓解前后的总体甲基化水平无明显差异。
Objective To investigate the relationship between the overall genomic methylation level and primary nephrotic syndrome in children. Methods Totally 20 children with primary nephrotic syndrome who were diagnosed as nephrotic syndrome in our hospital were enrolled as nephropathy group. Another 20 children with health checkup in our hospital were collected as healthy control group. The levels of total nephropathy The level of methylation changes. Results The overall methylation level in nephropathy group was (0.343 ± 0.054)%, while that in healthy control group was (0.870 ± 0.116)%. The difference between the two groups was statistically significant (P <0.01). At the same time, the overall methylation level of nephropathy in 18 children with steroid-induced nephrotic syndrome was detected before and after remission. The overall level of methylation before nephropathy was (0.340 ± 0.058)%, and the level of total methylation after remission was (0.395 ± 0.049)%, there was no significant difference between the two groups (P> 0.05). Conclusion The overall level of methylation in children with primary nephrotic syndrome is decreased. There is no significant difference in the overall level of methylation before and after nephropathy in children with steroid-induced nephrotic syndrome.