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建立了基于一种快速、高效分离与测定五味子中五味子甲素、五味子乙素和五味子酯甲含量的胶束毛细管电泳-紫外检测分析新方法。以毛细管(70cm×50μm i.d×375μm o.d)为分离通道,在最佳电泳介质(30mmol/L SDS,pH=7.2,20mmol/L磷酸盐缓冲溶液)中,当分离电压为21kV时,3种待测物质在11min内达到基线分离。在254nm波长处,五味子甲素、五味子乙素和五味子酯甲的线性响应范围分别为5.0—150.0μg/mL、1.0—100.0μg/mL和1.5—150.0μg/mL;检出限分别为1.0、0.22μg/mL和0.4μg/mL。该方法具有良好的重现性,以100.0μg/mL的五味子甲素、五味子乙素和五味子酯甲测定其保留时间和峰高相对标准偏差(RSD)(n=7)均小于2.98%。本方法用于中药五味子中五味子甲素、五味子乙素和五味子酯甲的含量测定,结果令人满意。
A rapid and efficient method for the separation and determination of Schisandra chinensis, Schisandrin B and Schisandra chinense A was developed. The new method was developed for the determination of Schisandra chinensis. Using capillary (70cm × 50μm id × 375μm od) as separation channel, in the best electrophoresis medium (30mmol / L SDS, pH = 7.2 and 20mmol / L phosphate buffer solution), when the separation voltage is 21kV, Substances measured in 11min to baseline separation. The linear response range of Schisandrin, Schisandrin B and Schisandra chinensis was 5.0-150.0μg / mL, 1.0-100.0μg / mL and 1.5-150.0μg / mL respectively at the wavelength of 254nm. The detection limits were 1.0, 0.22 μg / mL and 0.4 μg / mL. The method has good reproducibility. The retention time and relative standard deviation of peak height (RSD) (n = 7) were all less than 2.98% with 100.0μg / mL Schisandrin, Schisandrin B and Schisandra. The method is applied to the determination of schisandrin A, Schisandrin B and schisandrin A in Chinese schisandra chinensis with satisfactory results.