论文部分内容阅读
目的:对副溶血性弧菌快速测试片的检测性能进行测试研究。方法:以副溶血性弧菌、霍乱弧菌、溶藻弧菌等49株标准和参考菌株检测测试片的敏感性、特异性和可重复性。结果:该测试片对纯菌的检测灵敏度达到8 cfu/m l;与霍乱弧菌等17种非目的菌无交叉反应;重复性试验结果相对偏差低于8%;24 h可完成检验工作。应用该测试片检测人工染菌样品和自然样品,检测结果与GB或SN标准方法一致。结论:副溶血性弧菌快速测试片具有敏感度高、特异性强、重复性好、操作简便等优点,可用于食品和水产品中副溶血性弧菌的快速初筛。
Objective: To test the detection performance of Vibrio parahaemolyticus rapid test strip. Methods: The sensitivity, specificity and repeatability of test strips were tested with 49 standard and reference strains of Vibrio parahaemolyticus, Vibrio cholera and Vibrio alginolyticus. Results: The sensitivity of the test strip to pure bacteria was 8 cfu / ml. There was no cross-reaction with 17 non-objective bacteria such as Vibrio cholerae. The relative deviation of the repeatability test was less than 8%. The testing work could be completed within 24 hours. Application of the test piece to detect artificial bacterial and natural samples, test results and GB or SN standard method. Conclusion: Vibrio parahaemolyticus rapid test piece has the advantages of high sensitivity, strong specificity, good repeatability, easy operation and so on. It can be used in rapid screening of Vibrio parahaemolyticus in food and aquatic products.