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采用静电纺制备聚丙烯腈/纳米蒙脱土(PAN/MMT)复合纳米纤维膜,将其置于漆酶溶液中用于漆酶的固定,再将固定有漆酶的纳米纤维膜用于多酚氧化物的催化与吸附。采用扫描电镜(SEM)对PAN和PAN/MMT纤维膜进行观察;采用可见光分光光度计研究酶的温度稳定性、酸碱稳定性、存储稳定性和重复使用稳定性;并用可见光分光光度计测量不同反应时间的多酚溶液。结果表明:固定化漆酶的温度稳定性、pH稳定性、以及存储稳定性均有一定程度的提高;在5次重复试验后,固定化酶的活性仍高于原始活性的50%;多酚物质被漆酶有效催化,而最终反应物被PAN/MMT复合纤维膜吸收。
Electrospun polyacrylonitrile / nano-montmorillonite (PAN / MMT) composite nanofibrous membrane was prepared by electrospinning, placed in laccase solution for laccase immobilization, and laccase-immobilized nanofiber membrane was used in more Catalytic and Adsorption of Phenol Oxides. Scanning electron microscopy (SEM) was used to observe the PAN and PAN / MMT fiber membranes. The temperature stability, acid-base stability, storage stability and reuse stability of the enzyme were studied by visible light spectrophotometer. Reaction time polyphenol solution. The results showed that the temperature stability, pH stability and storage stability of the immobilized laccase increased to a certain extent. The activity of the immobilized enzyme was still higher than 50% of the original activity after 5 repeated experiments. The material is effectively catalyzed by laccase and the final reactant is absorbed by the PAN / MMT composite fiber membrane.