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目的探讨天然低水平放射性辐射对氧化损伤及其修复基因表达水平的影响。方法选择广东省阳西县天然放射性高本底辐射地区(HBRA)和恩平市天然放射性低本底辐射地区(CA)各48名50~58岁土生土长男性居民为研究对象,按年龄匹配,佩戴热释光剂量计测得外照射剂量。采集外周血并分离血浆和白细胞,ELISA法测定血浆8-羟基脱氧鸟苷(8-OHd G)的含量,QPCR法测定血白细胞人8-羟基鸟嘌呤核苷酸酶修复基因(Human Mut Thomologue,h MTH1)m RNA转录水平。结果 HBRA组和CA组居民外周血血浆中8-OHd G浓度分别为(0.27±0.11)μg/m L和(0.32±0.10)μg/m L,差异有统计学意义(P<0.05),与个人剂量呈负相关(rs=-0.271,P=0.013);h MTH1 m RNAΔCt值分别为(9.61±2.51)和(10.01±1.24),差异无统计学意义(P>0.05),但与个人剂量呈负相关(rs=-0.219,P=0.028)。结论长期暴露于天然放射性低本底辐射地区可降低居民氧化损伤水平、提高DNA损伤修复能力。
Objective To investigate the effect of natural low level radioactive radiation on oxidative damage and the expression of its repair gene. Methods Forty-eight native Chinese 50- to 58-year-old native male and female residents in natural radionuclide radiation area (HBRA) and natural radiation low background radiation area (CA) in Yangxi County of Guangdong Province were selected as research objects. External dose measured by the dosimeter. Peripheral blood was collected and the plasma and leukocytes were separated. The content of 8-OHdG in plasma was measured by ELISA. The level of 8-OHG in human leukocyte was detected by QPCR method (Human Mut Thomologue, h MTH1) m RNA transcription level. Results The concentrations of 8-OHd G in peripheral blood of residents in HBRA group and CA group were (0.27 ± 0.11) μg / m L and (0.32 ± 0.10) μg / m L, respectively, with statistical significance (P <0.05) (Rs = -0.271, P = 0.013); h MTH1 m RNAΔCt values were (9.61 ± 2.51) and (10.01 ± 1.24) respectively, with no significant difference (P> 0.05) Was negatively correlated (rs = -0.219, P = 0.028). Conclusion Long-term exposure to low background radioactive natural radiation can reduce the resident level of oxidative damage and improve DNA damage repair ability.