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目的 探讨海风藤新木脂素类成分对缺血鼠脑细胞间粘附分子 1(ICAM 1)及其mRNA表达的影响。方法 建立大鼠大脑中动脉闭塞 /再灌注模型 ,应用RT PCR及免疫组织化学的方法 ,观察海风藤新木脂素复合物以及银杏内脂对缺血鼠脑ICAM 1及其mRNA表达的影响。结果 免疫组织化学染色显示ICAM 1在假手术组鼠脑呈低表达 ;缺血 90min表达上调 ;缺血 90min再灌 2 4h后ICAM 1表达较前差异有非常显著性 (P <0 .0 1) ;而海风藤新木脂素类成分以及银杏内脂治疗组于相同时限鼠脑ICAM 1表达与单纯缺血、缺血再灌注组比较显著下调(P <0 .0 1)。假手术组鼠脑ICAM 1mRNA呈低表达 ;缺血 90min/再灌 2 4h其表达水平较对照组显著上调 (P <0 .0 1) ;药物治疗组于相同时限ICAM 1mRNA的表达显著下调 (P <0 .0 1)。结论 海风藤新木脂素类成分以及海风藤酮能明显降低缺血脑组织ICAM 1及其mRNA的表达 ,从而减轻脑缺血后炎性病理损害 ,达到脑保护作用
Objective To investigate the effect of the components of sea vine lignans on the expression of intercellular adhesion molecule-1 (ICAM 1) and mRNA in ischemic rats. Methods The middle cerebral artery occlusion/reperfusion model in rats was established. RT-PCR and immunohistochemical methods were used to observe the effects of kaempferolum lignan complex and ginkgo lactone on the expression of ICAM 1 and mRNA in the brain of ischemic rats. Results Immunohistochemical staining showed that ICAM 1 was lowly expressed in the sham-operated rat brain; the expression of ischemic myocardium was up-regulated after 90 min; the expression of ICAM 1 was significantly different from the previous one after 90 min ischemia and 24 h after reperfusion (P < 0.01). However, the expression of ICAM-1 in the rat brain at the same time was significantly down-regulated compared with the ischemic and ischemic reperfusion group (P < 0.01). The expression of ICAM 1 mRNA in the sham-operated rats brain was low; the expression of ICAM 1 mRNA was significantly down-regulated in the cerebral ischemic group at 90 min/reperfusion 24 h (P < 0.01); the expression of ICAM 1 mRNA was significantly down-regulated in the drug-treated group at the same time (P <0.01). <0. 0 1). Conclusion The new lignans and sea rotenone of Eupatorium can significantly reduce the expression of ICAM 1 and its mRNA in ischemic brain tissue, thus reducing the inflammatory pathological damage after cerebral ischemia and achieving brain protection.