论文部分内容阅读
目的探讨TLR7基因多态性与尖锐湿疣易感性及复发的相关性。方法采用Snapshot方法对140例尖锐湿疣患者及105例对照组外周静脉血中TLR7 rs179008、rs2302267和rs864058基因多态性进行了检测,并对尖锐湿疣组及对照组间上述基因位点的基因频率差异进行χ2检验,同时对治疗后6个月复发及无复发的尖锐湿疣患者间进行了基因频率差异的χ2检验,P<0.05为差异有统计学意义。结果TLR7rs179008和rs864058仅在复发的尖锐湿疣组患者中各检测出1例杂合突变。尖锐湿疣组TLR7 rs2302267基因中TT,TG和GG分别为109、22和9例,对照组分别为98、5和2例,尖锐湿疣组突变基因G频率显著高于对照组,差异有统计学意义(χ2=13.33,P<0.01),而且复发尖锐湿疣组突变基因G频率显著高于无复发尖锐湿疣组,差异有统计学意义(χ2=5.83,P<0.05)。结论 TLR7 rs2302267基因多态性不仅与尖锐湿疣易感性相关,而且与尖锐湿疣治疗后的复发相关。
Objective To investigate the relationship between polymorphism of TLR7 gene and the susceptibility and recurrence of condyloma acuminatum. Methods The gene polymorphisms of TLR7 rs179008, rs2302267 and rs864058 in peripheral blood of 140 patients with condyloma acuminatum and 105 controls were detected by the Snapshot method. The differences of the gene frequencies among the genital warts and controls Χ2 test was carried out. At the same time, χ2 test was performed for the frequency of genotypes between patients with and without recurrent condyloma acuminatum at 6 months after treatment. P <0.05 was considered statistically significant. Results One case of heterozygous mutation was detected in TLR7 rs179008 and rs864058 only in patients with condyloma acuminatum. The TT, TG and GG of TLR7 rs2302267 gene in condyloma acuminatum group were 109, 22 and 9 cases respectively, while the control group were 98, 5 and 2 cases respectively. The frequency of G gene in condyloma acuminatum group was significantly higher than that in control group, the difference was statistically significant (χ2 = 13.33, P <0.01), and the frequency of G gene mutation in condyloma acuminata group was significantly higher than that in non-recurrence condyloma acuminatum group (χ2 = 5.83, P <0.05). Conclusion TLR7 rs2302267 gene polymorphism is not only associated with susceptibility to condyloma acuminatum, but also with the relapse after condyloma acuminatum treatment.