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目的 探讨红景天对 AD转基因小鼠行为的影响和抗氧化应激反应的神经保护作用。方法 用 APP- C1 0 0转基因小鼠和健康(NTg)鼠模型来观察单剂红景天 8w抗氧化效果。Open field活动用来评估转基因鼠和健康鼠的移动、探索能力及焦虑状态。 Western印迹法定量分析转基因鼠血清及大脑 Cn/ Zn SOD1、APP及 Aβ蛋白含量。免疫组织化学法观察转基因鼠大脑皮层和海马的病理改变。结果 与 NTg鼠比较 ,转基因鼠通过格子次数 (grid cross)和排泄物 (粪尿 )均显著增加 (P<0 .0 5)。通过 Western印迹法及密度定量测定 ,红景天治疗组的转基因鼠 Cn/ ZnSOD1蛋白含量在血清及大脑中较对照组明显减少 (P<0 .0 5)。大脑 APP、βCTF及 Aβ蛋白含量无变化 ,免疫组化在大脑皮层和海马未见 Aβ沉积。结论 红景天可通过减少 SOD1氧化蛋白的含量 ,抑制转基因鼠血清及大脑的氧化应激反应水平
Objective To investigate the effect of Rhodiola on the behavior of AD transgenic mice and the neuroprotective effect of anti-oxidative stress. Methods The anti-oxidation effect of a single dose of Rhodiola rosea 8w was observed in APP-C100 transgenic mice and healthy (NTg) mouse models. The Open field activity was used to assess the mobility, exploration ability, and anxiety state of transgenic mice and healthy mice. Western blot analysis was performed to quantitatively analyze the levels of Cn/Zn SOD1, APP and Aβ protein in transgenic mice serum and brain. The histopathological changes of cerebral cortex and hippocampus in transgenic mice were observed by immunohistochemistry. Results Compared with NTg mice, the transgenic mice significantly increased both the grid cross and fecal matter (P<0.05). Western blotting and densitometric determination showed that the protein content of Cn/ZnSOD1 in the transgenic mice of the Rhodiola treatment group was significantly reduced in the serum and brain compared to the control group (P<0.05). There was no change in APP,βCTF, and Aβ protein levels in the brain. Immunohistochemistry showed no deposition of Aβ in the cerebral cortex and hippocampus. Conclusion Rhodiola can reduce the level of oxidative stress in serum and brain of transgenic mice by reducing the content of SOD1 protein.