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目的 提高抗表皮生长因子受体（ＥＧＦＲ） 单抗放免显像的诊断效果。方法 应用三步法（ 抗ＥＧＦＲ单抗生物素亲和素，ＩｇＭ生物素，１１１ＩｎＤＴＰＡ生物素）给药，在注射放射性标记物后２ ～４ｈ 对荷Ａ５４９ 肿瘤细胞裸鼠进行γ显像并测定生物学分布。结果 三步法组２ ｈ 已见肿瘤部位放射性浓聚，４ ｈ 明显显影；对照组无明显显影。三步法组显像瘤／血比值为２－３５４ ±０－４３２ ，明显高于对照组（ 两步法组、放射性标记物组和直接标记法组分别为１－４１５ ±０－３３２，０－９６１ ±０－４４６ ，１－４３８ ±０－３６１） ，Ｐ＜０－０５。结论 三步法给药能迅速降低本底，提高靶／ 非靶比值，缩短显像时间，改善显像质量
Objective To improve the diagnostic value of anti-EGFR monoclonal antibody radiosurgery. Methods The three-step method (anti-EGFR monoclonal antibody biotin avidin, IgM biotin, 111In DTPA biotin) administration, 2 ~ 4h after injection of radiolabel on A549 tumor cells nude mice γ imaging and determination of biological distribution. Results The radioactive concentration of the tumor site was observed in the three-step method at 2 h and evidently at 4 h. There was no obvious development in the control group. The tumor-to-blood ratio in the three-step method group was 2-354 ± 0-432, which was significantly higher than that in the control group (1-415 ± 0-332, 0 for the two-step method group, the radiolabel group, -961 ± 0-446, 1-438 ± 0-361), P <0-05. Conclusion The three-step method can rapidly reduce the background, improve the target / non-target ratio, shorten the imaging time and improve the imaging quality