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研究以籼稻品种9311为受体亲本,粳稻品种越光为供体亲本构建染色体片段置换系(CSSLs)并对RVA谱特征值进行QTL定位,为稻米蒸煮食味品质的遗传改良创造理想条件。研究利用612对SSR标记对亲本(越光和9311)进行多态性筛选,使用检测出的多态性引物对染色体片段置换系株系进行鉴定。共鉴定出138个株系,并构成染色体片段置换系。该染色体片段置换系的水稻全基因组覆盖率达到89%,置换系群体中超过80%的株系携带的供体亲本染色体片段数少于5个,其中单片段置换系为51个,比例为36.95%。利用该群体共定位到位于6条不同染色体上9个区域的10个QTL,表型贡献率范围为8.85%~42.65%,其中q BDV-1、q PT-4.1、q PT-4.2和q CSV-3等4个QTL为首次报道。q HPV-2位于第2染色体上标记RM341与RM525之间,q BDV-2位于第2染色体RM341与RM1385标记之间,与该染色体上支链淀粉合成有关基因等位。本研究构建的CSSLs及定位的控制RVA谱特征值相关QTL,为稻米蒸煮食味品质的研究创造了有利条件。
In this study, we constructed a chromosome segmental replacement line (CSSLs) with indica rice 9311 as the parent and the japonica rice variety Koshihikari as donor parent and mapped the QTLs of RVA profiles to create ideal conditions for genetic improvement of rice cooking and eating quality. In this study, 612 pairs of SSR markers were used to screen the parents (Koshihikari and 9311) for polymorphism. Chromosome segment substitution lines were identified using the detected polymorphic primers. A total of 138 lines were identified and constituted a chromosomal segment replacement line. The whole genome coverage of rice chromosomes was 89%, and more than 80% of the substitution lines carried fewer than 5 donor chromosomes, of which 51 were single-fragment substitutions with a ratio of 36.95 %. Using this population, 10 QTLs located in 9 regions of 6 different chromosomes were co-localized and their phenotypic contribution rates ranged from 8.85% to 42.65%. Among them, q BDV-1, q PT-4.1, q PT-4.2 and q CSV -3 and other four QTLs for the first time reported. q HPV-2 is located on chromosome 2 between markers RM341 and RM525, and q BDV-2 is located between markers RM341 and RM1385 on chromosome 2 and is allelic to the gene encoding amylopectin synthesis on this chromosome. The CSSLs constructed in this study and the QTLs for controlling the eigenvalues of RVA profiles are favorable for the research on the eating and cooking qualities of rice.