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酶法制备头孢氨苄的转化液中有一些与头孢氨苄结构相似的底物和前体等物质。为了从转化液中分析头孢氨苄的含量，我们设计并建立了用反相ＨＰＬＣ法分析测定头孢氨苄的效价，并及时反馈转化液、吸附废液、解析液、固体粗品中头孢氨苄的含量，为中间体各环节的质量控制提供了一个简便、快速、灵敏、准确的方法。实验采用Ｗａｔｅｒｓ液相色谱仪，Ｃ１８柱，流动相为甲醇、水、冰乙酸混合液，在２～６０μｇ／ｍｌ浓度范围内线性关系良好，ｒ＝０．９９９９（ｎ＝６），平均回收率为９９．５９％，ＲＳＤ为０．５３％。
Enzymatic preparation of cephalexin in the transformation of some of the cephalexin structure similar to the substrate and precursor substances. In order to analyze the content of cephalexin from the conversion solution, we designed and established the titer of the determination of cephalexin by reverse phase HPLC, and timely feedback the content of cephalexin in the conversion liquid, adsorption waste liquid, analytical liquid and solid crude product, For all aspects of intermediate quality control provides a simple, fast, sensitive and accurate method. The experiment was performed on a Waters liquid chromatograph with a C18 column with a mobile phase of methanol, water and glacial acetic acid with a good linearity (r = 0.9999 (n = 6)) in the range of 2 ~ 60μg / ml. The average recoveries 99.59%, RSD 0.53%.