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目的 :观察TECA型组合型人工肝支持系统 (HALSS)治疗急性肝功能衰竭 (ALF)患者的有效性和安全性。方法 :分离中国实验用小型猪肝细胞并培养于BALSS的生物反应器中。患者的血浆先经碳吸附或血浆置换后 ,循环流经BALSS的中空纤维管。每次治疗持续 6～ 7h。观察治疗前、后患者生理和血液生化指标的变化。结果 :从每只小型猪的肝脏平均可得到 (1.0～ 3.0 )× 10 10 个猪肝细胞 ,存活率 >85 %。TECA -HALSS治疗结束后 ,患者的血NH3、ALT、AST、TB、DB等水平明显降低 ;PTA水平升高 ;患者的神志由嗜睡或昏迷转为清醒。治疗中患者生命体征平稳、血电解质水平无明显改变。结论 :TECA -HALSS是一种能迅速、安全、有效地为ALF患者提供暂时的肝功能支持有效途径
Objective: To observe the effectiveness and safety of TECA combined artificial liver support system (HALSS) in the treatment of patients with acute liver failure (ALF). Methods: Isolation of Chinese experimental miniature pig hepatocytes and culture in BALSS bioreactor. The patient’s plasma was first subjected to carbon adsorption or plasma exchange and then circulated through the hollow fibers of the BALSS. Each treatment lasts 6 ~ 7h. Observed before and after treatment of patients with changes in physiological and blood biochemical indicators. Results: The average number of liver cells (1.0-3.0) × 10 10 piglets per mini pig was 85%. After the treatment of TECA -HALSS, the levels of NH3, ALT, AST, TB and DB in the patients decreased significantly; the level of PTA increased; the consciousness of the patients turned from drowsiness or coma to awake. In patients with stable vital signs, blood electrolyte levels did not change significantly. CONCLUSIONS: TECA -HALSS is an effective, rapid, safe and effective way to provide transient liver function support for ALF patients