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该文通过Time-lapse技术,以无电场作用的人肺腺癌细胞Calu-3细胞为对照组,以分别暴露于电场强度为2 V/cm、4 V/cm和6 V/cm的Calu-3细胞为实验组,研究了外源微直流电场对Calu-3迁移的影响。结果表明,Calu-3细胞可通过单细胞和群体细胞两种形式迁移,直流电场对这两种迁移形式具有显著的影响:无电场作用时,单细胞和群体性的Calu-3随机迁移;加入直流电场后,单细胞和群体性的Calu-3朝向负极定向迁移,迁移方向性指数、迁移方向持续性指数、轨迹速度、位移速度显著高于无电场作用的对照组。电场强度为4 V/cm时,单细胞和群体性的Calu-3趋电性最明显,迁移方向最恒定。相同强度的电场作用下,Calu-3群体细胞迁移的方向性指数和方向持续性均高于单细胞迁移,但单细胞迁移的轨迹速度和位移速度快于群体细胞迁移。
By using Time-lapse technique, Calu-3 cells of human lung adenocarcinoma cells without electric field as control group were exposed to Calu-3 with electric field of 2 V / cm, 4 V / cm and 6 V / 3 cells as experimental group to study the effect of exogenous micro-DC electric field on the migration of Calu-3. The results showed that Calu-3 cells could migrate through both single-cell and multi-population cells, and the DC field had a significant effect on these two migration forms: single cell and population Calu-3 migrated randomly without electric field; After DC field, single cell and population Calu-3 migrated toward the negative direction, the migration direction index, migration direction continuity index, trajectory velocity and displacement velocity were significantly higher than those of the control group without electric field. When the electric field intensity is 4 V / cm, the single cell and population Calu-3 have the most obvious electrification and the migration direction is the most constant. Under the same electric field strength, the directionality and directionality of cell migration of Calu-3 population were higher than single cell migration, but the single cell migration path velocity and displacement speed faster than the population cell migration.