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目的:筛选适合分离纯化辣椒叶总黄酮的一种大孔树脂,同时用响应面法进行优化得到最佳纯化工艺。方法:采用热回流法提取辣椒叶总黄酮,以吸附率和解吸率为考察指标,考察6种不同型号的大孔树脂(HPD100、HPD450、HPD600、HPD826、D101、AB-8)对辣椒叶总黄酮的吸附能力与解吸能力,确定最佳树脂。通过动态吸附解吸实验考察此树脂对辣椒叶总黄酮的最佳分离纯化工艺。结果:通过对辣椒叶总黄酮吸附分离性能的分析显示HPD600为最佳树脂,最优工艺为:上样浓度为10 mg/mL,上样量为10 mL,洗脱体积为4 BV,洗脱液流速为4 mL/min,洗脱液pH为7,依次用水、10%、30%乙醇冲洗树脂柱,50%乙醇为洗脱液。纯化后的黄酮纯度435.4 mg/g。结论:该方法简便,操作简单,对辣椒叶总黄酮的纯化效果较好。
OBJECTIVE: To screen a macroporous resin suitable for the isolation and purification of total flavonoids from pepper leaves, and to optimize the process by response surface methodology. Methods: Total flavonoids were extracted from hot pepper leaves by hot reflux method. The adsorption rate and desorption rate were used as indexes to investigate the effects of six kinds of macroporous resins (HPD100, HPD450, HPD600, HPD826, D101 and AB- Flavone adsorption capacity and desorption ability to determine the best resin. Through dynamic adsorption and desorption experiments, the optimal separation and purification of total flavonoids from pepper leaves was investigated. Results: According to the analysis of the adsorption and separation properties of total flavonoids in capsicum leaves, HPD600 was the best resin. The optimum conditions were as follows: the sample concentration was 10 mg / mL, the sample volume was 10 mL and the elution volume was 4 BV The liquid flow rate was 4 mL / min, the eluent pH was 7, the column was washed with water, 10%, 30% ethanol, and 50% ethanol as eluent. Purified flavonoids purity 435.4 mg / g. Conclusion: The method is simple and easy to operate, and the purification of flavonoids from pepper leaves is better.