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1975年作者首先报道无细胞的腹腔液具有抗菌活性,因为正常妇女的腹腔液对酵母菌、革兰氏阳性和阴性需氧菌具有制菌和杀菌效果。近来,Fromkes发现7例患Laennec肝硬化者的腹水对革兰氏阴性杆菌,包括脆弱杆菌也有抗菌活性。作者对28例因不育、月经过多、原发性闭经或腹痛,但无感染、恶性或全身性疾病的妇女,作腹腔镜检查时,常规地取2～12ml腹腔液;另又在4例卵巢癌、4例胃肠道癌、1例乳癌和2例肝炎后肝硬化的病人中,作腹腔穿刺,取得25～200ml腹水。这些病例在近一个月内均末用过抗菌素。将标本离心,以除去小颗粒物质。然后取0.1ml放入10ml的肉汤中,于37℃培养48小时;其余
In 1975, the authors first reported that cell-free peritoneal fluid has antibacterial activity because normal women’s peritoneal fluid has a bacteriocidal and bactericidal effect on yeast, Gram-positive and negative aerobic bacteria. Recently, Fromkes found that ascites in seven patients with Laennec cirrhosis also had antibacterial activity against Gram-negative bacilli, including Bacteroides fragilis. The author of 28 cases of infertility, menorrhagia, primary amenorrhea or abdominal pain, but no infection, malignant or systemic disease for laparoscopy, routinely take 2 ~ 12ml peritoneal fluid; the other in 4 Cases of ovarian cancer, 4 cases of gastrointestinal cancer, 1 case of breast cancer and 2 cases of posthepatitic cirrhosis patients, abdominal puncture, obtained ascites 25 ~ 200ml. Antibiotics have not been used in these cases in nearly a month. Centrifuge the specimen to remove small particles. Then take 0.1ml into 10ml of broth, at 37 ℃ for 48 hours; the rest