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本文比较了三种具有6-O-酸基-酰基团的胞壁酰二肽(MDP)类似物〔6-O-C_(14)-O-(C_(16))-(MDP)(Me)、6-O-C_(14)-O-(C_(12))-(MDP)(Me)和6-O-C_(14)-O-(C_(14))-(MDP)(Me)〕水溶液的佐荆活性,以6-O-B30-MDP为阳性对照.将佐剂(100μg/mg)分别与HBsAg(100μg/mg)、流感病毒血凝素疫苗(9μg/ml)或破伤风类毒素(31μg/ml)等体积混合作为免疫抗原,对8～10周龄CDF1小鼠作腹腔注射(每组8只,每只0.2ml),3周后进行加强免疫.从眼眶后静脉或心脏采血,用ELISA法测定血清中IgG滴度.
In this paper, we compared three muramyl dipeptide (MDP) analogues with 6-O-acyl-acyl groups [6-O-C 14 -O- (C 16) - (Me) and 6-O-C (14) -O- (C 14) - (MDP) (Me )〕 Aqueous solution of the activity of ZOU to 6-O-B30-MDP as a positive control.The adjuvant (100μg / mg) were respectively HBsAg (100μg / mg), influenza virus hemagglutinin vaccine (9μg / ml) (31 μg / ml) as an immunological antigen, 8 to 10-week-old CDF1 mice were injected intraperitoneally (8 per group, 0.2 ml each) and boosted after 3 weeks. Or heart blood, serum IgG titer was measured by ELISA.