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【目的】昆虫适应新环境的能力与其对温度的耐受能力密切相关。热激蛋白HSP70和HSP90具有提高生物体温度耐受性的功能。烟粉虱Bemisia tabaci(Gennadius)不同隐种和不同种粉虱对温度的适应能力有差异,这与它们的热激蛋白基因拷贝数的差异可能相关。【方法】利用实时荧光定量PCR方法,检测入侵型烟粉虱MED隐种和MEAM1隐种、本地型烟粉虱AsiaⅡ1隐种以及温室粉虱Trialeurodes vaporariorum(Westwood)基因组DNA中热激蛋白基因hsp70和hsp90的拷贝数。【结果】不同种类的粉虱和烟粉虱不同隐种体内的hsp70和hsp90的含量均有较大差异,其中hsp70和hsp90拷贝数在入侵型烟粉虱MED和MEAM1隐种中含量较其他两种均高,而在土著种AsiaⅡ1隐种中含量最低,在温室粉虱中居中。此外,相同物种雌雄成虫hsp70和hsp90的拷贝数也不同,雌虫hsp70和hsp90拷贝数约为雄虫的2倍。【结论】不同种粉虱及烟粉虱不同隐种的hsp70和hsp90的拷贝数可能与其耐热性差异相关。本研究为解释不同种粉虱、烟粉虱不同隐种及其不同性别的耐热性差异机制提供了进一步的依据。
【Objective】 The ability of insects to adapt to the new environment is closely related to their tolerance to temperature. Heat shock proteins HSP70 and HSP90 have the function of increasing the temperature tolerance of the organism. Bemisia tabaci (Gennadius) has different temperature adaptability to different species and different species of whiteflies, which may be related to their differences in the copy number of heat shock proteins. 【Method】 The real-time fluorescent quantitative PCR method was used to detect the Hb70 and Hsp70 in the genomic DNAs of MED and MEAM1 invaders and native species of Bemisia tabacles, as well as in the genomic DNA of Trialeurodes vaporariorum (Westwood) hsp90 copy number. 【Result】 The contents of hsp70 and hsp90 in different species of whitefly and whitefly Bemisia tabaci were significantly different, and the copy number of hsp70 and hsp90 in the invasive species of B. tabaci MED and MEAM1 was higher than that of the other two The species was the highest, while it was the lowest in the hidden species of the native species, Asia Ⅱ 1, and was intermediate in the greenhouse whitefly. In addition, the copy number of hsp70 and hsp90 of male and female adults of the same species is also different, and the copy number of hsp70 and hsp90 of female is about 2 times that of male. 【Conclusion】 The copy number of hsp70 and hsp90 in different species of whiteflies and whiteflies may be related to their difference in heat tolerance. This study provides a further basis for explaining the different heat tolerance mechanisms of different species of whitefly and whitefly, and their different sexes.