论文部分内容阅读
目的检测口腔鳞状细胞癌(OSCC)患者淋巴结标本中细胞角蛋白19(CK19)的表达情况,寻找发现OSCC淋巴结转移及微转移的有效分子生物学手段。方法应用荧光定量RT-PCR检测8例OSCC患者的119枚淋巴结标本中CK19mRNA表达水平,并与HE染色及免疫组化进行比较。结果 HE染色及免疫组化同时检测到CK19阳性淋巴结24枚,荧光定量RT-PCR检测到CK19阳性者48枚,HE染色及免疫组化结果CK19阴性而定量RT-PCR阳性的微转移淋巴结主要分布于颈Ⅱ区。淋巴结总体微转移率为20.17%。荧光定量RT-PCR检测的CK19阳性淋巴结与HE染色及免疫组化比较有统计学差异(x~2=22.04,P<0 01)。结论对于OSCC淋巴结转移与微转移的检测,荧光定量RT-PCR对CK19 mRNA的检测较HE染色和免疫组化敏感,分子病理检测方法的应用可以有助于肿瘤的分期判断、预后评估及治疗方案的抉择。
Objective To detect the expression of cytokeratin 19 (CK19) in lymph node specimens of patients with oral squamous cell carcinoma (OSCC) and to find effective molecular biological means to find out the lymph node metastasis and micrometastasis in OSCC. Methods The expression of CK19 mRNA in 119 lymph node specimens from 8 patients with OSCC was detected by real-time fluorescence quantitative RT-PCR, and compared with HE staining and immunohistochemistry. Results Twenty-four CK19-positive lymph nodes were detected by HE staining and immunohistochemistry. 48 were positive for CK19 by fluorescence quantitative RT-PCR. The results of HE staining and immunohistochemistry were negative for CK19 and mainly for quantitative RT-PCR positive micrometastases In the neck Ⅱ area. The overall rate of lymph node micrometastasis was 20.17%. Fluorescent quantitative RT-PCR detection of CK19 positive lymph nodes and HE staining and immunohistochemistry were statistically significant (x ~ 2 = 22.04, P <0.01). Conclusions For the detection of lymph node metastasis and micrometastasis in OSCC, the detection of CK19 mRNA by fluorescence quantitative RT-PCR is more sensitive than HE staining and immunohistochemistry, and the application of molecular pathological detection method can be helpful for the staging of tumor, prognosis evaluation and treatment plan The choice.