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目的观察红芪多糖对免疫抑制大鼠免疫功能与细胞因子的影响。方法SD大鼠腹腔注射环磷酰胺80 mg·kg~(-1),1次/天,连用3 d,构建免疫抑制模型大鼠。从造模成功后的模型大鼠中选取20只,按性别、体重随机分为2组(每组10只):模型组和实验组;另设10只健康大鼠为空白组。实验组每只大鼠按100 mg·kg~(-1)·d-1剂量灌服相应药物,模型组及空白组每只大鼠灌服等体积的蒸馏水,1次/天,连续7 d。给药7 d后,各组大鼠禁食8 h,股动脉取血,用酶联免疫法检测血清中免疫球蛋白Ig G、Ig M及Ig A的含量;用同法检测血清中细胞因子肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ)、白细胞介素-2(IL-2)和IL-4的含量。结果给药后,空白组、模型组与实验组大鼠的Ig G分别是(0.98±0.50),(0.28±0.18),(0.60±0.28)μg·m L~(-1);这3组的Ig M分别是(365.21±26.85),(267.03±47.89),(286.22±30.03)μg·m L~(-1);这3组的Ig A分别是(79.52±9.30),(57.88±10.44),(69.19±14.18)μg·m L~(-1)。与空白组比较,模型组差异均有统计学意义(均P<0.05);与模型组比较,实验组差异有统计学意义(均P<0.05)。空白组、模型组与实验组大鼠的TNF-α分别是(39.52±5.28),(70.14±6.68),(51.16±5.60)pg·m L~(-1);这3组的IFN-γ分别是(24.31±5.86),(10.88±3.17),(15.52±3.92)pg·m L~(-1);这3组的IL-2分别是(261.51±46.20),(153.7±34.96),(185.84±17.74)pg·m L~(-1);这3组的IL-4分别是(18.91±2.56),(9.32±3.86),(15.23±2.75)pg·m L~(-1)。与空白组比较,模型组差异有统计学意义(均P<0.05);与模型组比较,实验组差异有统计学意义(均P<0.05)。结论红芪多糖能提高环磷酰胺所致免疫抑制模型大鼠的免疫功能。
Objective To observe the effect of Radix Hedysari Polysaccharide on immune function and cytokines in immunosuppressed rats. Methods SD rats were injected intraperitoneally with cyclophosphamide (80 mg · kg -1, once daily for 3 days) to establish immunosuppressed model rats. Twenty healthy rats were selected and divided into two groups randomly according to their sex and weight (10 in each group): model group and experimental group; another 10 healthy rats were selected as blank group. The rats in the experimental group were dosed with the corresponding drugs at a dose of 100 mg · kg -1 · d -1. The rats in the model group and the blank group were dosed with distilled water in an equal volume for 1 d, . After 7 days of administration, rats in each group were fasted for 8 hours and their femoral arteries were taken for blood. Serum immunoglobulin Ig G, Ig M and Ig A levels were detected by enzyme-linked immunosorbent assay. Serum levels of cytokines Tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-2 (IL-2) and IL-4. Results After administration, the Ig G of the blank group, the model group and the experimental group were (0.98 ± 0.50), (0.28 ± 0.18) and (0.60 ± 0.28) μg · m L -1, respectively IgM were (365.21 ± 26.85), (267.03 ± 47.89), (286.22 ± 30.03) μg · m L -1, respectively; Ig A in these three groups were (79.52 ± 9.30), (57.88 ± 10.44) ), (69.19 ± 14.18) μg · m L -1. Compared with the blank group, there were significant differences in the model group (all P <0.05). Compared with the model group, the difference in the experimental group was statistically significant (P <0.05). The levels of TNF-α in the blank group, the model group and the experimental group were (39.52 ± 5.28), (70.14 ± 6.68) and (51.16 ± 5.60) pg · m L -1, respectively. The levels of IFN-γ (24.31 ± 5.86), (10.88 ± 3.17) and (15.52 ± 3.92) pg · m L -1, respectively. The levels of IL-2 in these three groups were (261.51 ± 46.20) and (153.7 ± 34.96) (185.84 ± 17.74) pg · m L -1. The IL-4 levels of these three groups were (18.91 ± 2.56), (9.32 ± 3.86) and (15.23 ± 2.75) pg · m L -1 . Compared with the blank group, the difference between the model group was statistically significant (P <0.05). Compared with the model group, the experimental group had significant difference (all P <0.05). Conclusion Radix Hedysari Polysaccharide can improve immune function of immunosuppressed rats induced by cyclophosphamide.