论文部分内容阅读
本实验对小白鼠阴栓龄胚第9～16天的前、中肾发育过程中的组织学和组织化学变化进行了核酸(甲绿-哌若咛稀染法)、PAS 反应(McManus 法)、AcP(硫化铅法)、ATPase(Wach-stein-Meisel 中性法)、AlP(钙钴法)、SDH(Pearse’s MTT 法),酯酶(Pearson 法)观察,以成年鼠肾作对照。1.小白鼠前肾(阴栓龄胚第9～10.5天),除含丰富的 RNA 外,其余指标无明显的阳性反应。2.小白鼠中肾(早期胚第10.5～13天),中肾小管及导管除含丰富的 RNA 外,AlP、AcP、ATPase、SDH、酯酶几无活性,PAS 反应极弱。中肾后期 RNA 减少,PAS 反应、AcP、ATP-ase、AlP 活性增强,并随中肾小管、中肾导管及密勒氏管之继续发育或退化而有强弱不同。特别是 AcP 在所有退化组织中均明显增强,对其意义也予以讨论。
In this experiment, the histological and histochemical changes during the development of pronucleus and middle kidney of mice aged from the 9th to the 16th day were observed. The results of the nucleic acid (A green-Pirogamycin dilution method), PAS reaction (McManus method) , AcP (lead sulfide method), ATPase (Wach-stein-Meisel neutral method), AlP (calcium cobalt method), SDH (Pearse’s MTT method) and esterase (Pearson method). 1. Mouse anterior kidney (embryo age of the first eighteen to 10.5 days), in addition to rich RNA, the other indicators no significant positive reaction. In mice, kidney (early embryo 10.5 ~ 13 days), renal tubules and catheters in addition to rich RNA, AlP, AcP, ATPase, SDH, esterase few activity, PAS very weak. In the late metaphase, RNA decreased, PAS reaction, AcP, ATP-ase, AlP activity increased, and with the renal tubules, mesonephros and Miller tube continued development or degeneration and have different strengths and weaknesses. In particular, AcP was significantly enhanced in all degenerative groups, and its meaning is also discussed.