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应用Ames试验,微核试验,精子细胞畸形试验,体外染色体畸变试验和细胞转化试验对变构蛇神经毒素(MN-81)进行研究。实验结果表明:变构蛇神经毒素(MN-81)对TA97,TA98,TA100及TA102在加和不加S9条件下均无致突性,以1/2,1/5,1/10LD_(50)剂量诱发小鼠骨髓多染红细胞的微核率与阴性对照无显著差异(P<0.05)以12.5,10μg/ml剂量在加S9时诱发的染色体畸变率与阴性对照相差显著(P<0.05),各剂量组加和不加S9时诱发N1H3T3细胞转化率与性阴对照相差非常显著(P<0.01)以42.21mg/kg剂量对雄性小鼠精子已明显诱发出致畸效应。表明变构蛇神经毒素(MN-81)具有潜在致突性作用。
Ames test, micronucleus test, sperm cell deformity test, in vitro chromosomal aberration test and cell transformation assay were used to study allosteric neurotoxin (MN-81). The results showed that allosteric zaparin neurotoxin (MN-81) had no abrogation on TA97, TA98, TA100 and TA102 with and without S9, with 1/2, 1/5 and 1/10 LD50 (P <0.05). There was a significant difference (P <0.05) in the rate of chromosome aberration induced by S9 at doses of 12.5 and 10 μg / ml compared with the negative control (P <0.05) (P <0.01), the spermatozoa of male mice had evoked a teratogenic effect obviously with the dose of 42.21mg / kg, the conversion rates of N1H3T3 cells induced by each dose group and without S9 were significantly different (P <0.01). Suggesting that allosteric zaparin neurotoxin (MN-81) has a potential role in the process of mutagenesis.