Construction and expression of SET gene and siRNA recombinant adenovirus vectors

• 作 者: 许波群 陆品红 李瑛 薛凯 李梅 马翔 刁飞扬 崔毓桂 刘嘉
• 机 构: DepartmentofClinicalReproductiveMedicine,DepartmentofObstetricsandGynecology,JiangsuProvinceFamilyPl
• 出 处: 生殖医学杂志
• 发表日期: 2010年A02期
• 关键词: 重组腺病毒载体 SIRNA 基因片段 逆转录聚合酶链反应 绿色荧光蛋白 巨细胞病毒 RNA干扰 293细胞 Adenovirus vector Homolog
• 基金项目: Founds： Projects from China National Department of Science and Technology （81070465）, and a project from China Jiangsu Prov ince （BM2008151） AcknowledgementsThis study was supported by projects from C

<正>Objective:To construct SET gene recombinant adenovirus vector and SET gene small interfering RNA(SiRNA) recombinant adenovirus vector for over-expression or knock-down of SET levels. Methods:The cDNA sequence of SET was cloned by reverse transcri