Qualitative Study of Three Cell Culture Methods

• 作 者: 王爱国 夏涛 冉鹏 陈学敏 Andreas K.Nuessl
• 机 构: DepartmentofEnvironmentalHealth,DepartmentofSurgery
• 出 处: 华中科技大学学报：医学英德文版
• 发表日期: 2002年4期
• 关键词: 肝细胞 单层培养 夹层培养 细胞色素P4501A 生物反应器 酶渗漏 白蛋白分泌 monolayer culture sandwich culture bior

Summary: Primary rat hepatocytes were cultured using different in vitro models and the enzymeleakage, albumin secretion, and cytochrome P450 lA (CYP lA) activity were observed. The re-sults showed that the level of LDH was decreased over time in culture.