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目的 探讨黄芪甲苷(astragaloside Ⅳ,AS-Ⅳ)对高糖刺激下人晶状体上皮(human lens epithelium,HLE)细胞氧化应激的影响.方法 采用40 mmol·L-1的葡萄糖培养HLE细胞建立细胞氧化损伤模型,用不同浓度的黄芪甲苷干预,CCK-8比色法检测细胞活力,倒置显微镜观察细胞形态并拍照,Hoechst-PI双染法观察细胞核凋亡,H2DCFDA检测细胞内活性氧(reactive oxygen species,ROS)水平,Western blot检测caspase-3,caspase-9的表达情况.结果 CCK-8结果显示,用5μmol·L-1、10 μmol·L-1黄芪甲苷处理后,HLE细胞存活率分别提高到(60.86±3.11)％和(80.67±3.70)％,与高糖组(43.65±3.43)％比较差异具有统计学意义(P＜0.05);细胞形态学观察结果及Hoechst-PI双染法结果显示,黄芪甲苷能够抑制HLE细胞凋亡;H2DCFDA荧光探针染色结果显示,黄芪甲苷处理后HLE细胞内ROS生成量下降,氧化损伤细胞减少;此外,黄芪甲苷可以抑制高糖诱导的HLE内caspase-3及caspase-9的表达,其抑制效应与剂量正相关.结论 黄芪甲苷可以抑制高糖诱导的人HLE细胞氧化损伤及凋亡的发生,对糖尿病性白内障具有预防作用.“,”Objective To investigate the effect of astragaloside Ⅳ on high glucose-induced human lens epithelial (HLE) cells from oxidative stress.Methods HLE cells oxidative damage model was induced by 40 mmol· L-1 glucose,and intervented with different concentrations of astragaloside Ⅳ,cell survival rate was detected by CCK-8 colorimetry,cell morphology were observed and detected by inverted microscope,nucleus apoptosis was observed by Hoechst-PI double staining,intracellular reactive oxygen species (ROS) were detected by H2DCFDA staning,the expression of caspase-3 and caspase-9 were tested by Western blot.Results CCK-8 results showed that HLE cell survival rate increased to (60.86 ± 3.11) ％ and (80.67 ±3.70)％ after treated with 5 μmol · L-1、10 μmol · L-1 astragaloside Ⅳ,the difference was statistically significant compared with high glucose group (43.65 ± 3.43) ％,P ＜ 0.05;Cell morphological observation and Hoechst-PI double staining results showed that astragaloside Ⅳ inhibited HLE cells apoptosis.H2DCFDA fluorescent probe dying showed that astragaloside Ⅳ reduced ROS generation in HLE cells and decreasing oxidative damage cells;Besides,astragaloside Ⅳ inhibited the expression of high glucose-induced caspase-3 and caspase-9 expression in HLE cells and the inhibitory effect positively correlated with the dose.Conclusion Astragaloside Ⅳ could inhibit high glucose-induced oxidative damage and apoptosis in human HLE cells,which has preventive effect on diabetic cataract.