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以嗜酸氧化亚铁硫杆菌T.f_6优势菌株为原始菌,采用盐酸羟胺对其进行化学诱变,重点研究了化学诱变对菌种活性、生长繁殖以及尾矿浸出体系的影响.结果表明,羟胺诱变能使菌种产生明显变异,能很好地提高菌种活性和生物浸出能力.盐酸羟胺质量分数1.0%时所得诱变菌的氧化活性最高,32h后Fe~(2+)氧化率达到100%,而在相同条件下原始菌的Fe~(2+)氧化率达到100%则延迟32h.尾矿浸出30d,诱变菌的铜浸出率比原始菌提高了20.7%,比酸浸铜浸出率提高了85%;同时,到达浸出终点的时间比原始菌提前了5～8d.说明诱变菌浸出效果好于原始菌,远优于化学浸出.菌种诱变前后扫描电镜测试表明,诱变菌种的形态没有变化,但细胞大小有所改变,表面变得光滑且出现胞外分泌物,同时细胞间聚团现象明显.
The dominant strain of Thiobacillus ferrooxidans T.f_6 was used as the original strain, and its chemical mutagenesis was carried out with hydroxylamine hydrochloride, and the effects of chemical mutagenesis on the activity, growth and reproduction of tailings and leaching system of tailings were studied emphatically. , Hydroxylamine mutagenesis can produce significant variation of bacteria, can well improve the activity of bacteria and bioleaching ability.When hydroxylamine hydrochloride mass fraction of 1.0%, the highest mutagenic bacteria oxidation activity, 32h after Fe ~ (2+) oxidation Rate of 100%, while under the same conditions, the rate of Fe 2+ oxidation of the original bacteria was delayed by 32 h after 100% of the initial bacteria leaching.Thirty days after the leaching of tailings, the copper leaching rate of the mutants was increased by 20.7% Leaching rate of leaching copper increased by 85%; at the same time, reach the end of leaching time ahead of the original bacteria 5 ~ 8d, indicating that the leaching effect of mutagenic bacteria is better than the original bacteria, far superior to the chemical leaching. The results showed that there was no change in the morphology of the mutagenic bacteria, but the size of the cells changed, the surface became smooth and extracellular secretions appeared, and the phenomena of agglomeration between cells were obvious.