论文部分内容阅读
目的研究亚慢性染铝后对大鼠学习记忆及α-氨基-3-羟基-5-甲基-4-异恶唑丙酸(AMPA)受体基因表达的影响。方法无特定病原体级健康成年雄性SD大鼠36只,按体质量随机分为0.41、0.82、1.23 mg.kg-1.d-1麦芽酚铝[Al(mal)3]组和对照组。染毒组分别腹腔注射Al(mal)30.41、0.82、1.23 mg.kg-1.d-1,对照组给予等容量生理氯化钠溶液。染毒8周后,用Morris水迷宫实验检测大鼠空间学习记忆能力,实时荧光定量聚合酶链反应法检测大鼠海马组织AMPA受体亚单位GluR-1、GluR-2、GluR-3基因表达情况。结果水迷宫结果表明,与对照组比较,0.82、1.23mg.kg-1Al(mal)3组大鼠找到平台时间延长(P<0.05),并且目标象限停留时间缩短(P<0.05);1.23 mg.kg-1.d-1Al(mal)3组穿越平台次数减少(P<0.05)。基因检测表明,与对照组比较,3个Al(mal)3组GluR-1、GluR-2基因表达水平均降低(P<0.01);0.82、1.23 mg.kg-1.d-1Al(mal)3组GluR-3基因表达也降低(P<0.01)。结论亚慢性染铝可使大鼠学习记忆能力下降,其机制可能与大鼠海马AMPA受体各亚基基因表达改变有关。
Objective To investigate the effect of subchronic aluminum on the learning and memory and the gene expression of α -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor in rats. Methods Thirty-six healthy adult male Sprague-Dawley (SD) rats without specific pathogen were randomly divided into four groups according to the body weight of 0.41,0.82,1.23 mg.kg-1.d-1 Al (mal) 3 and the control group. Rats in the exposure group were given intraperitoneal injection of Al (mal) 30.41,0.82,1.23 mg.kg-1.d-1 respectively. The control group was given equal volume of physiological sodium chloride solution. After 8 weeks of exposure, spatial learning and memory abilities of rats were detected by Morris water maze test and GluR-1, GluR-2 and GluR-3 gene expressions of AMPA receptor subunits in hippocampus were detected by real-time fluorescence quantitative polymerase chain reaction Happening. Results The water maze results showed that compared with the control group, the platform time was prolonged (P <0.05) and the target quadrant residence time was shortened (P <0.05) in 0.82 and 1.23 mg.kg-1Al (mal) .kg-1.d-1Al (mal) 3 groups decreased across the platform (P <0.05). The results of gene test showed that the expression of GluR-1 and GluR-2 gene in three Al (mal) 3 groups were decreased (P <0.01) compared with the control group; 0.82,1.23 mg.kg-1.d-1Al GluR-3 gene expression was also decreased in 3 groups (P <0.01). Conclusion Subchronic aluminum exposure can decrease the learning and memory abilities of rats, which may be related to the changes of AMPA receptor subunit gene expression in hippocampus of rats.