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选用初始体质量约2.02 g的黄颡鱼(Pelteobagrus fulvidraco)960尾,随机分为6组,分别投喂6种用蝇蛆粉替代饲料中0%、20%、40%、60%、80%、100%鱼粉配制的等氮(39.5%)等能(16.8 MJ/kg)饲料,分别记为G0、G20、G40、G60、G80和G100。养殖期为60 d。结果显示,黄颡鱼肌肉水分、粗蛋白质、粗脂肪含量受蝇蛆粉替代鱼粉水平的影响不显著(P>0.05),但G60～G100灰分含量与G0相比显著升高(P<0.05)。蝇蛆粉替代鱼粉对肌肉pH、滴水损失(DL)、蒸煮损失(CL)和超氧化物歧化酶(SOD)活性影响不显著(P>0.05)。与G0相比,G20～G100肌肉胶原蛋白含量差异不显著(P>0.05),G40～G60肌肉丙二醛(MDA)含量显著降低(P<0.05)。与G0相比,G80肌肉精氨酸(Arg)、组氨酸(His)、苯丙氨酸(Phe)、必需氨基酸(TEAA)含量显著高(P<0.05),各替代组总氨基酸含量差异不显著(P>0.05)。各组肌肉谷氨酸(Glu)、甘氨酸(Gly)、丙氨酸(Ala)、天冬氨酸(Asp)等风味氨基酸和总风味氨基酸(TFAA)含量差异不显著(P>0.05)。肌肉C14:0、C18:3n-3、DHA、EPA+DHA、多不饱和脂肪酸(PUFA)、n-3 PUFA含量和n-3/n-6比值随蝇蛆粉替代鱼粉水平的升高而降低,其中G60～G100显著低于G0(P<0.05);C16:1、单不饱和脂肪酸(MUFA)含量随替代水平增加而升高,其中G60和G100显著升高(P<0.05)。结果表明,当蝇蛆粉替代鱼粉水平不超过40%时,除MDA含量显著降低外,蝇蛆粉对黄颡鱼肌肉常规成分、理化指标、氨基酸和脂肪酸含量影响不显著。
Ninety-six of Pelteobagrus fulvidraco with an initial body weight of about 2.02 g were randomly divided into 6 groups and fed with 6 kinds of maggot powder instead of 0%, 20%, 40%, 60%, 80% (39.5%) and other isoforms (16.8 MJ / kg) formulated with 100% fishmeal were recorded as G0, G20, G40, G60, G80 and G100, respectively. Breeding period of 60 d. The results showed that the content of water, crude protein and crude fat in muscle of Catfish were not significantly affected by fly meal replacement (P> 0.05), but the content of G60 ~ G100 was significantly higher than that of G0 (P <0.05) . The effect of maggot meal replacement fish meal on muscle pH, drip loss (DL), cooking loss (CL) and superoxide dismutase (SOD) activity was insignificant (P> 0.05). Compared with G0, there was no significant difference in G20 ~ G100 muscle collagen content (P> 0.05) and G40 ~ G60 muscle malondialdehyde (MDA) content was significantly lower (P <0.05). The contents of Arg, His, Phe and TEAA in G80 were significantly higher than those in G0 (P <0.05), and the contents of total amino acids in each substitution group were significantly different Not significant (P> 0.05). There was no significant difference in the contents of amino acids and total flavoring amino acids (TFAA) between Glu, Gly, Ala and Asp in each group (P> 0.05). The ratio of muscle C14: 0, C18: 3n-3, DHA, EPA + DHA, PUFA, n-3 PUFA content and n-3 / n-6 increased with the replacement of fishmeal (P <0.05); C16: 1, monounsaturated fatty acid (MUFA) content increased with the replacement level increased, G60 and G100 increased significantly (P <0.05). The results showed that the maggot powder had no significant effect on the regular component, physicochemical index, amino acid and fatty acid content of the muscle of yellow catfish, except that the content of maggot powder instead of fish meal did not exceed 40%.