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目的:检测甲胎蛋白(a-fetoprotein,AFP)基因RS12506899、RS16849384、RS7688274,三位点基因型在广西地区原发性肝细胞癌患者中的分布,探讨候选基因型对患者血清AFP水平以及临床病理指标的影响。方法:选取广西医科大学一附院和广西医科大学肿瘤医院2005年3月至2011年3月间行肝癌切除手术治疗肝细胞癌(hepatocellular carcinoma,HCC)患者177例。提取组织DNA,Taq-man法进行基因分型。对各组基因型间AFP水平及临床病理特点进行比较。结果:不同基因型各组间AFP水平、肿瘤直径、乙肝表面抗原的分布情况,差异无统计学意义。无肝硬化的HCC患者AFP水平高于合并肝硬化的HCC患者(P=0.047)。单因素生存分析结果提示P53抗体阳性程度越高,其无瘤生存时间越短(P=0.010)。结论:在本组人群中,AFP候选基因型与HCC患者的AFP水平及其临床参数不相关;P53阳性者预后较差。
OBJECTIVE: To detect the distribution of a-fetoprotein (AFP) gene RS12506899, RS16849384, RS7688274 and three-locus genotypes in patients with primary hepatocellular carcinoma in Guangxi and to investigate the relationship between the candidate genotypes and serum AFP levels The impact of pathological indicators. Methods: A total of 177 patients with hepatocellular carcinoma (HCC) underwent resection of hepatocellular carcinoma between March 1 and March 2011 in First Affiliated Hospital of Guangxi Medical University and Guangxi Medical University Cancer Hospital from March 2005 to March 2011. Tissue DNA was extracted and genotyped by Taq-man method. AFP levels and clinicopathological features of each genotype were compared. Results: There was no significant difference in AFP level, tumor diameter and hepatitis B surface antigen distribution among different genotypes. AFP levels were higher in HCC patients without cirrhosis than in HCC patients with cirrhosis (P = 0.047). The results of univariate survival analysis showed that the higher the positive degree of P53 antibody, the shorter the survival time of tumor-free (P = 0.010). CONCLUSION: In this cohort, AFP candidate genotypes are not associated with AFP levels and clinical parameters in HCC patients; P53 positive patients have poor prognosis.