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目的探讨维生素E(Vitamin E,VE)对二甲基甲酰胺(N,N-dimethylformamide,DMF)致急性肝损伤的拮抗作用。方法将50只健康SPF级雄性昆明小鼠随机分为5组,分别为正常对照组、VE(10 mg/kg)对照组、DMF中毒模型组及低(5 mg/kg)、高(10 mg/kg)剂量VE保护组,每组10只。VE对照组和低、高剂量VE保护组采用灌胃方式染毒VE溶液,正常对照组和DMF中毒模型组分别染毒等体积玉米油,每天1次,连续5 d;最后1次染毒VE后0.5 h,DMF中毒模型组及各剂量VE保护组一次性灌胃染毒2 g/kg的DMF溶液,同时,正常对照组和VE对照组给等体积蒸馏水。检测小鼠血清丙氨酸氨基转氨酶(ALT)、天冬氨酸氨基转氨酶(AST)和黄嘌呤氧化酶(XOD)活力,肝脏匀浆中谷胱甘肽(GSH)和脂质过氧化产物丙二醛(MDA)的含量。结果与DMF中毒模型组比较,VE保护组小鼠血清ALT、AST、XOD活力显著降低(P<0.05),肝脏GSH含量明显升高(P<0.05),MDA含量显著下降(P<0.05)。结论 VE对DMF引起的小鼠肝损伤具有拮抗作用。
Objective To investigate the antagonistic effect of vitamin E (VE) on acute liver injury induced by dimethyl formamide (DMF). Methods Fifty healthy SPF male Kunming mice were randomly divided into 5 groups: normal control group, VE (10 mg / kg) control group, DMF poisoning model group and low (5 mg / kg) / kg) dose of VE protection group, 10 in each group. VE control group and low and high dose VE protection group were treated by intragastric administration of VE solution, normal control group and DMF poisoning model group were infected with the same volume of corn oil, once a day for 5 consecutive days; the last dose of VE After 0.5 h, the DMF poisoning model group and each dose of VE protection group were given gavage 2 g / kg DMF solution at the same time. At the same time, the normal control group and the VE control group were given equal volume of distilled water. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and xanthine oxidase (XOD), glutathione (GSH) and lipid peroxidation Aldehyde (MDA) content. Results Compared with the DMF poisoning model group, the activities of ALT, AST and XOD in the VE protective group were significantly decreased (P <0.05), the content of hepatic GSH was significantly increased (P <0.05) and the content of MDA was significantly decreased (P <0.05). Conclusion VE can antagonize the liver injury induced by DMF in mice.