胃粘膜癌变过程中幽门螺杆菌感染与p53,c-erbB-2基因表达的研究

• 作 者: 郭长青 王玉萍 刘国永 马社旺 丁贯一 李继昌
• 机 构: 河南医科大学第一附属医院消化内科,河南医科大学第三附属医院病理科,开封医学专科学校第一附属医院消化科,中国长城铝业公司总医院
• 出 处: 世界华人消化杂志
• 发表日期: 1999年04期
• 关键词: 胃粘膜 癌变过程 幽门螺杆菌感染

目的探讨胃粘膜癌变过程中幽门螺杆菌（Ｈｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉ，Ｈｐ）感染与ｐ５３，ｃｅｒｂＢ２基因表达的关系．方法浅表性胃炎１６例，肠上皮化生２２例，异型增生１４例，早期胃癌１８例及进展期胃癌４０例作为研究对象．用ＷａｒｔｈｉｎＳｔａｒｙ银染色法检测Ｈｐ，用免疫组化Ｓｐ法检测ｐ５３和ｃｅｒｂＢ２的基因表达产物．结果Ｈｐ，ｐ５３，ｃｅｒｂＢ２在浅表性胃炎的检出率各为５００％，００％，００％；在肠上皮化生的检出率各为５９１％，２２７％，１３６％；在异型增生的检出率各为８５７％，６４３％，２８６％；在早期胃癌的检出率各为１６７％，３３３％，１１１％；在进展期胃癌的检出率各为５０％，５２５％，５５０％；在癌旁粘膜的Ｈｐ检出率为８６７％；在癌前病变中，Ｈｐ阳性组的ｐ５３，ｃｅｒｂＢ２表达率均高于Ｈｐ阴性组．结论Ｈｐ感染参与了胃癌前病变的发生与发展；Ｈｐ感染可引起野生型ｐ５３基因失活和ｃｅｒｂＢ２基因激活，从而导致胃粘膜的癌变．

Objective To investigate the relationship between Helicobacter pylori (Hp) infection and the expression of p53 and cerbB2 genes in gastric carcinogenesis. Methods 16 cases of superficial gastritis, 22 cases of intestinal metaplasia, 14 cases of dysplasia, 18 cases of early gastric cancer and 40 cases of advanced gastric cancer were studied. Hp was detected by Warthin-Stary silver staining and p53 and cerbB2 gene expression products were detected by immunohistochemical Sp method. Results The detection rates of Hp, p53, cerbB2 in superficial gastritis were 500%, 00%, and 00% respectively. The detection rate of intestinal metaplasia was 591. %, 22  7%, 13  6%; the detection rate of dysplasia was 85  7%, 64  3%, and 28  6% respectively; the detection rate of early-stage gastric cancer was 16  7%, respectively. 33%, 11% and 1% respectively; the detection rate of advanced gastric cancer was 50%, 52%, and 55%, respectively; the detection rate of Hp in adjacent mucosa was 86.7%. In precancerous lesions, the expression rates of p53 and cerbB2 in Hp-positive group were higher than those in Hp-negative group. Conclusion Hp infection is involved in the occurrence and development of precancerous lesions. Hp infection can lead to the inactivation of wild-type p53 gene and activation of cerbB2 gene, leading to the carcinogenesis of gastric mucosa.