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本实验采用一年生珍珠半夏球茎为外植体,接种在MS+KT(1.0μM)+NAA(0.4μM)+蔗糖(3.0%)+琼脂(0.7%)培养基中,可在20~25 d内完成芽的萌发。再生芽在MS+NAA(0.4μM)+蔗糖(3.0%)+琼脂(0.7%)培养基中完成芽的伸长。伸长芽在MS+KT(1.0μmol/L)+NAA(0.4μmol/L)+蔗糖(3.0%)+琼脂(0.7%)培养基中,60 d内可以完成生根。将根放入1/2MS+NAA(2.0~5.0μmol/L)+IBA(2.0~5.0μmol/L)培养基中可完成生根。为建立稳定的高频离体快繁体系和适宜于遗传转化的再生体系,我们运用RAPD检测技术来确定离体培养过程中材料的体细胞遗传变异情况。本研究为山东省珍珠半夏的大规模生产和半夏的遗传改良提供技术平台。
In this experiment, annual pearl bulbs were used as explants and inoculated on MS + KT (1.0μM) + NAA (0.4μM) + sucrose (3.0%) + agar Complete bud germination. Regeneration buds Bud elongation was accomplished in MS + NAA (0.4 μM) + sucrose (3.0%) + agar (0.7%) medium. The elongation buds could be rooted within 60 d in MS + KT (1.0 μmol / L) + NAA (0.4 μmol / L) + sucrose (3.0%) + agar (0.7%) medium. The rooting could be done by rooting into the medium of 1 / 2MS + NAA (2.0 ~ 5.0μmol / L) and IBA (2.0 ~ 5.0μmol / L) In order to establish a stable high-frequency in vitro propagation system and a suitable regeneration system for genetic transformation, we used RAPD to determine the somatic genetic variation of the materials during in vitro culture. This study provides a technological platform for the mass production of pearl pinellia in Shandong Province and the genetic improvement of Pinellia ternata.