Cloning of Chicken Anemia Virus vp3 Gene and Apoptosis Inductive Effect of vp3 Gene In Vitro

• 作 者: 孙军 王宇哲 宗义强 屈伸
• 机 构: DepartmentofBiochemistryandMolecularBiology
• 出 处: 华中科技大学学报：医学英德文版
• 发表日期: 2003年4期
• 关键词: 鸡贫血病毒 克隆 VP3基因 VP3基因 诱导效应 细胞凋亡 PCR 肿瘤治疗学 chicken anemia virusgene cloningapoptos
• 基金项目: 湖北省自然科学基金

Using PCR technique, the vp3 gene of chicken anemia virus (CAV) was cloned into the eukaryotic expression vector pcDNA3 to construct a recombinant pcDNA-vp3. Restriction enzyme digestion and sequencing analysis revealed that CAV vp3 gene was correctly ins