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目的 :观察胰岛素样生长因子 1(IGF 1)对新生大鼠低氧缺血性脑损伤 (Hypoxic ischemicbraindam age ,HIBD)的治疗作用。方法 :制作新生大鼠HIBD模型 ,把HIBD大鼠分成IGF 1治疗组 (n =6)和非治疗组 (n= 6) ,HIBD后 72h取脑标本、病理切片 ,予HE染色 ,观测两组大鼠大脑皮层梗死面积率、海马坏死细胞率 ,用Tunel法测定凋亡细胞率。结果 :与非治疗组相比 ,IGF 1治疗组的皮层梗死面积率、皮层凋亡细胞率、海马坏死细胞率 ,均显著降低 (P <0 .0 5)。但两组海马凋亡细胞率差别无显著性。结论 :基因重组人类IGF 1(rhIGF 1)治疗新生鼠HIBD疗效明显
Objective: To observe the therapeutic effect of insulin-like growth factor-1 (IGF-1) on hypoxic ischemic brain injury (HIBD) in neonatal rats. Methods: HIBD model was established in neonatal rats. HIBD rats were divided into IGF1 treatment group (n = 6) and non-treatment group (n = 6). The HIBD rats were sacrificed at 72 hours after HIBD. The area of cerebral cortex infarction area and the percentage of necrotic cells in hippocampus were determined by Tunel method. Results: Compared with the untreated group, the area of infarction, cortical apoptotic cells and hippocampal necrotic cells in IGF1 group were significantly decreased (P <0.05). However, there was no significant difference in apoptotic cells between the two groups. Conclusion: The effect of recombinant human IGF 1 (rhIGF 1) on neonatal HIBD is obvious