论文部分内容阅读
目的:探讨虎杖对肾缺血/再灌(ischemia/reperfusion,I/R)注损伤大鼠肾脏的保护作用及其机制。方法:健康雄性SD大鼠72只随机分为4组,假手术组、缺血再灌注组、虎杖及虎杖苷(PD)预处理组。采用切除大鼠右侧肾脏,左侧肾蒂夹闭60min肾I/R模型。在缺血前10min、缺血再灌注6、12h后分别检测血清肌酐(Scr)、血清尿素氮(BUN)、肾组织丙二醛(MDA)水平及超氧化物歧化酶(SOD)活性,观察肾组织的病理变化,并分别进行比较。结果:虎杖及PD预处理组肾组织病理变化轻于肾缺血再灌注组,与假手术组比较,在不同时间点肾缺血再灌注组的Scr、BUN、MDA水平明显升高(P<0.05),SOD活性明显降低(P<0.05)。虎杖及PD预处理组的Scr、BUN、MDA水平均较肾I/R组明显降低(P<0.05),而SOD活性明显增强(P<0.05)。结论:在肾缺血再灌注损伤的过程中,虎杖可增强SOD活性,减少MDA,改善肾功能,其中PD起了主要作用但还有其他成分起作用。
Objective: To investigate the protective effect of Polygonum cuspidatum on renal injury induced by ischemia/reperfusion (I/R) in rats and its mechanism. METHODS: Seventy-two healthy male SD rats were randomly divided into 4 groups: sham operation group, ischemia-reperfusion group, Polygonum cuspidatum and Polydatin (PD) pretreatment group. The right kidney was removed from the rat and the left renal pedicle was clipped for 60 min renal I/R model. Serum creatinine (Scr), serum urea nitrogen (BUN), malondialdehyde (MDA) and superoxide dismutase (SOD) activity were measured 10 min before ischemia and 6 and 12 h after ischemia and reperfusion. Pathological changes in kidney tissue were compared separately. Results: The renal pathological changes of Polygonum cuspidatum and PD pretreatment group were lighter than that of renal ischemia reperfusion group. Compared with sham operation group, the levels of Scr, BUN and MDA in renal ischemia-reperfusion group were significantly higher at different time points (P< 0.05), SOD activity was significantly reduced (P <0.05). The levels of Scr, BUN and MDA in the Polygonum cuspidatum and PD pretreatment group were significantly lower than those in the renal I/R group (P<0.05), while the SOD activity was significantly increased (P<0.05). Conclusion: During the course of renal ischemia-reperfusion injury, Polygonum cuspidatum can enhance SOD activity, reduce MDA, and improve renal function. Among them, PD plays a major role but other components play a role.