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研究人胰岛素样生长因子Ⅱ（ＩＧＦ－Ⅱ）反义ＲＮＡ对肝癌细胞株ＳＭＭＣ－７７２１的抑制效应。方法人ＩＧＦ－ⅡｃＤＮＡ０．１ｋｂ反向插人真核细胞表达载体ｐｃＮＡ３，获得ＩＧＦ－Ⅱ反义ＲＮＡ表达载体ｐＩＧＦ－ⅡＡＳ，将其导人人肝癌细胞株ＳＭＭＣ-７７２１，观察软琼脂培养集落形成的能力。流式细胞仪（ＦＣＭ）测定ｐＩＧＦ－ⅡＡｓ表达ＩＧＦ－Ⅱ反义ＲＮＡ对ＳＭＭＣ-７７２１细胞生长的影响。结果转导人ｐＩＧＦ－ⅡＡｓ的ＳＭＭＣ－７７２１细胞，不能在软琼脂上形成集落，ＦＣＭＭ实导人ＰＩＧＦ－ⅡＡｓ细胞Ｓ期增多，而对照组ＳＭＭＣ－７７２１细胞和带ＤＣＤＮＡ３空载体的ＳＭＭＣ－７７２１细胞则无明显变化．结论ｐＩＧＦ-ⅡＡＳ＆义ＲＮＡ体外可抑制肝癌细胞株ＳＭＭＣ－７７２１的致瘤性。
To study the inhibitory effect of human insulin-like growth factor II (IGF-II) antisense RNA on hepatoma cell line SMMC-7721. Methods The human IGF-II cDNA 0.1 kb was inserted into the eukaryotic expression vector pcNA3, and the IGF-II antisense RNA expression vector pIGF-IIAS was obtained, which was then introduced into human hepatoma cell line SMMC-7721 to observe the formation of soft agar cultured colonies. ability. Flow cytometry (FCM) was used to determine the effect of pIGF-IIAs-expressed IGF-II antisense RNA on the growth of SMMC-7721 cells. RESULTS: Human SMMC-7721 cells transduced with pIGF-IIAs could not form colonies on soft agar. FCMM actually increased S phase in human PIGF-IIAs cells, but SMMC-7721 cells in control group and SMMC-7721 cells with DCDNA3 empty vector. There is no obvious change. Conclusion The pIGF-IIAS/sense RNA can inhibit the tumorigenicity of hepatocellular carcinoma cell line SMMC-7721 in vitro.