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本研究借助生物信息学分析从苹果基因组数据库中获得了6个含全长编码序列的Ca2+/H+反向转运蛋白(Ca2+/H+antiporter,CAX)基因,分别命名为MdCAX1、MdCAX2、MdCAX3、MdCAX4、MdCAX5和MdCAX6。基因和蛋白结构特性分析结果显示:6个CAX在基因结构和蛋白特性等方面存在差异;MdCAXs隶属于I-A型和I-B 2个亚型,均含有N-端自我抑制区(N-terminal regulatory region,NRR)、CaD功能域、C-端功能域、C功能域和D功能域。荧光定量PCR分析表明,MdCAX1在‘富士’苹果叶片中表达量表现为基部叶片>中部叶片>顶端叶片,在果皮和果肉中的表达量低于叶片;MdCAX2在果皮和果肉中表达量高于叶片;MdCAX3在叶片和果实中的表达模式与MdCAX1相似,但在叶片中的表达量低于MdCAX1;MdCAX4在各被检测样本中表达量无明显变化;MdCAX5和MdCAX6在果皮和果肉组织中的表达量随发育进程呈逐渐升高趋势。叶片中Ca2+含量在不同叶位叶片中差异显著,果皮和果肉中Ca2+含量随着果实发育均呈下降趋势。上述研究结果表明:6个MdCAXs基因在基因结构、蛋白特性以及表达模式上存在差异,MdCAX1和MdCAX3基因表达与‘富士’苹果叶片和果实Ca2+含量变化密切相关。
In this study, six Ca2 + / H + antiporter (CaX + / H + antiporter, CAX) genes with full-length coding sequence were obtained from apple genome database by bioinformatics analysis and named as MdCAX1, MdCAX2, MdCAX3, MdCAX4 , MdCAX5 and MdCAX6. Gene and protein structural characteristics analysis showed that: 6 CAX gene structure and protein characteristics differences; MdCAXs belong to IA and IB 2 subtypes, both containing N-terminal regulatory region (N-terminal regulatory region, NRR), CaD domain, C-terminal domain, C domain and D domain. Quantitative real-time PCR analysis showed that the expression level of MdCAX1 in ’Fuji’ apple leaves showed that the expression level of MdCAX1 was lower than that of the leaves in the middle of the leaves> the middle leaves> the top leaves. The expression level of MdCAX2 in the peel and pulp was higher than that of the leaves . The expression pattern of MdCAX3 in leaves and fruits was similar to that of MdCAX1, but the expression level of MdCAX3 in leaves was lower than that of MdCAX1. The expression level of MdCAX4 in all tested samples showed no significant change. The expression of MdCAX5 and MdCAX6 in pericarp and pulp tissues With the development process was gradually increased. The content of Ca2 + in leaves was significantly different in leaves with different leaf positions. The content of Ca2 + in peel and pulp showed a decreasing trend with the development of fruits. The above results showed that the six MdCAXs genes were different in their gene structure, protein characteristics and expression patterns. The expression of MdCAX1 and MdCAX3 genes was closely related to the content of Ca2 + in ’Fuji’ apple leaves and fruits.