论文部分内容阅读
本文以一、二年生的水仙(Narcissus tazetta var.chinensis Roem.)的鳞茎为外植体,通过不同切割方式和两种培养基进行组培试验。试验结果表明,小鳞茎的形态发生有两种途径:带茎盘的二年生鳞茎切块和一年生鳞茎外层切块,可以在鳞片间的茎盘组织表层直接形成芽原基,然后发育成小鳞茎;不带茎盘的鳞片切块和带茎盘的一年生鳞茎的内层切块,都要先形成愈伤组织,由愈伤组织团分化出芽原基,再发育成小鳞茎。两种培养基对小鳞茎发育途径的影响无明显差异。组织学观察表明,直接从茎盘表层发生的芽原基,不是培养前原来就有的,而是在切割损伤刺激下,由茎盘表层组织脱分化形成的。
In this paper, the bulbs of Narcissus tazetta var. Chinensis Roem. Were used as explants for tissue culture experiments by different cutting methods and two media. The results showed that there are two ways for the morphogenesis of bulblets: the perennial bulbs with stem disks and the outer bulbs of annual bulbs can form bud primordia directly on the surface of the tissue between the scales, and then develop into small bulbs ; Scale cuttings without stem disks and inner cuttings of annual bulbs with stem disks must first form callus, differentiated from the callus tissue bud primordia, and then developed into bulblets. There was no significant difference between the two media on the development of bulblets. Histological observations showed that the bud primordium that occurs directly from the surface of the stem disc was not originally present prior to culture but was dedifferentiated by the surface tissue of the stem disc under the stimulation of the cutting injury.