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本文通过丙烯腈对大鼠肝脏Ｃａ２＋－ＡＴＰａｓｅ和磷酸化酶Ａ活性影响的研究，探讨了丙烯腈对动物肝脏钙稳态的影响，进一步阐明丙烯腈的毒作用机制。采用成年雄性ＳＤ大鼠，每天经口染毒，染毒剂量为０、１０、３０和５０ｍｇ／ｋｇ，连续染毒４２天，于染毒第１４天、２８天和４２天时分别测定各组肝匀浆Ｃａ２＋－ＡＴＰａｓｅ和磷酸化酶Ａ的活性。结果显示，丙烯腈可明显地抑制肝脏Ｃａ２＋－ＡＴＰａｓｅ（Ｐ＜０．０１），同时可显著地激活磷酸化酶Ａ的活性，特别是高剂量组（５０ｍｇ／ｋｇ）和染毒４２天时影响最明显，相关关系分析表明，丙烯腈对Ｃａ２＋－ＡＴＰａｓｅ和磷酸化酶Ａ的活性的影响均存在较好的剂量－反应和时间－反应关系（Ｐ＜０．０１）。结果认为丙烯腈能显著地抑制大鼠肝脏Ｃａ２＋－ＡＴＰａｓｅ和激活磷酸化酶Ａ的活性，可导致大鼠肝脏钙稳态的失调。
In this paper, the effect of acrylonitrile on the activity of Ca2 + -ATPase and phosphorylase A in rat liver was investigated to investigate the effect of acrylonitrile on the hepatic calcium homeostasis in animal and to further elucidate the toxic mechanism of acrylonitrile. Adult male Sprague-Dawley rats were orally administered with oral doses of 0, 10, 30 and 50 mg / kg orally daily for 42 days. On the 14th, 28th and 42th days of exposure, Homogenize Ca2 + -ATPase and phosphorylase A activity. The results showed that acrylonitrile could significantly inhibit the hepatic Ca2 + -ATPase (P <0.01) and activate phosphorylase A significantly, especially in high-dose group (50mg / kg) and 42-day exposure Obviously, the correlation analysis showed that there was a good dose-response and time-response relationship between acrylonitrile activity and Ca2 + -ATPase and phosphorylase A activity (P <0.01). The results showed that acrylonitrile can significantly inhibit the activity of rat liver Ca2 + -ATPase and activated phosphorylase A, can lead to calcium homeostasis in rat liver.