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甘蓝型油菜是由甘蓝与白菜型油菜杂交,染色体自然加倍而成.与甘蓝亲本相比,甘蓝型油菜的遗传资源狭窄,利用甘蓝资源是拓宽甘蓝型油菜遗传背景的方式之一,但是甘蓝与甘蓝型油菜杂交存在生殖隔离.利用29份甘蓝与1份自然甘蓝型油菜杂交;利用12份自然甘蓝型油菜,8份人工合成甘蓝型油菜,48份新型甘蓝型油菜和95份人工合成甘蓝型油菜同自然甘蓝型油菜杂交的DH系与1份甘蓝杂交,期望筛选出亲和性高的甘蓝或者甘蓝型油菜.对7份甘蓝与甘蓝型油菜柱头识别反应和胚珠发育观察,发现甘蓝花粉能够在甘蓝型油菜柱头上正常萌发、伸长和生长,但是授粉10d后胚珠开始败育,直到30d完全死亡.尽管29份甘蓝与甘蓝型油菜杂交平均结实率低(36.00%),但是一份野生甘蓝K8934与甘蓝型油菜的结实率能达到148.60%.4种不同类型的甘蓝型油菜与甘蓝杂交均表现低的可交配性,平均结实率差(10.90%).说明了从变异广泛的甘蓝材料中,可能筛选到与甘蓝型油菜杂交亲和性高的甘蓝材料.
Brassica napus was crossed with Brassica campestris and the chromosomes were doubled naturally.Compared with the parents of cabbage, the genetic resources of Brassica napus were narrow, and the utilization of cabbage resources was one of the ways to broaden the genetic background of Brassica napus, Brassica napus hybridized with reproductive isolation, using 29 parts of cabbage and 1 part of the natural Brassica napus hybridization; using 12 parts of natural Brassica napus, 8 parts of synthetic Brassica napus, 48 new Brassica napus and 95 parts of synthetic cabbage type The cross between rapeseed and natural Brassica napus was crossed with 1 part of cabbage, and it was expected that the high affinity cabbage or Brassica napus could be screened.It was observed that the stigma identification and ovule development of 7 parts of Brassica napus and Brassica napus Ovules germinated, elongated and grew normally in the stigma of Brassica napus, but the ovules began to abort after 10 days of pollination and died completely till 30d. Although the average seed setting rate of 29 cabbages crossed with Brassica napus was low (36.00%), The seed setting rate of Brassica napus K8934 and Brassica napus reached 148.60% .4 different types of Brassica napus hybrids with cabbage showed low malleability and average knot The real rate difference (10.90%) indicated that it was possible to screen kale with high affinity with Brassica napus from wide variety of cabbage materials.