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目的观察膀胱肿瘤患者的细胞免疫状态及卡介苗(BCG)、白细胞介素(IL-2)联合膀胱腔内灌注对其影响。方法用~3H-TdR掺入法测定自然杀伤细胞(NK)活性,免疫荧光法测定T淋巴细胞亚群,BCG、IL-2灌注前后动态观察,进行对比研究。结果膀胱肿瘤病人外周血NK活性,T_4细胞,T_4/T_8比值明显低于对照组,经BCG、IL-2膀胱灌注治疗后,NK活性,T_4细胞,T_4/T_8比值增高。NK活性,T_4/T_8的基础水平及灌注后的变化与膀胱癌复发有一定关系。结论膀胱肿瘤患者细胞免疫功能低下,膀胱腔内灌注BCG、IL-2能增强机体的免疫能力。
Objective To observe the cellular immunity status of bladder cancer patients and the effect of BCG and interleukin-2 (IL-2) combined with intravesical perfusion. Methods The activity of natural killer (NK) cells was measured by ~3H-TdR incorporation method. The T lymphocyte subsets were measured by immunofluorescence, and the dynamic changes observed before and after perfusion of BCG and IL-2 were compared. Results The peripheral blood NK activity, T_4 cells and T_4/T_8 ratio in bladder cancer patients were significantly lower than those in the control group. After BCG and IL-2 bladder instillation treatment, the NK activity, T_4 cells and T_4/T_8 ratio increased. The basal levels of NK activity, T_4/T_8 and changes after perfusion were related to the recurrence of bladder cancer. Conclusions The function of cellular immunity is low in bladder cancer patients. Intravesical BCG and IL-2 infusion can enhance the body’s immunity.