【摘 要】
：
Aim:TREK-1 (TWIK-related K+ channel-1) is a 2-pore-domain K+ channel subtype.The present study investigated the role of TREK-1 in cell death induced by oxidativ
【机 构】
：
Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Bei
论文部分内容阅读
Aim:TREK-1 (TWIK-related K+ channel-1) is a 2-pore-domain K+ channel subtype.The present study investigated the role of TREK-1 in cell death induced by oxidative stress.Methods:The cell viability of wild-type Chinese hamster ovary (CHO) and TREK-1-transfected CHO cells (TREK-1/CHO cells) was measured using 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay in the presence of sodium nitroprusside (SNP) or hydrogen peroxide (H2O2).Apoptosis of wild-type CHO and TREK-I/CHO cells was detected using Hoechst33342 staining.Results:Both SNP and H2O2 caused dose- and time-dependent growth inhibition of wild-type CHO and TREK-1/ CHO cells.Following a 12 h exposure to SNP,the 50% inhibition (IC50) values for wild-type CHO and TREK-1/CHO cells were calculated as 0.69 mmol/L and 1.14 mmol/L,respectively.The IC50 values were 0.07 mmol/L and 0.09 mmol/L in H2O2-treated wild-type CHO and TREK-I/CHO cells,respectively,following 12 h exposure to H2O2.Moreover,SNP/H2O2 induced less apoptosis in TREK-1/ CHO cells than that in wild-type CHO cells (P<0.05).Conclusion:The results demonstrated that TREK-1 played a protective role against oxidative injury.
其他文献
Aim: To develop a simple method for monitoring protein localization of epidermal growth factor (EGF) in living cells. Methods: Enhanced green fluorescent protei
无机营养在作物干物质中所占的比重较小，但对作物所起的作用却不容忽视，尤其是氮、磷、钾对作物产量和品质的影响更为重要。鉴于对专用强筋小麦品种8901的产量和品质形成与施肥的关系及配方施肥研究较少，笔者于2002—2003年度在河北农业大学教学基地进行了2项试验研究：以专用强筋小麦品种8901—11(简称8901)、高产品种石4185(简称4185)、高蛋白品种河农341(简称341)、耐早高产品种河
Aim: Angiotensin Ⅱ is believed to play an important role in tissue repair and remodeling in lungs by the angiotensin type Ⅰ (ATI) receptor via a number of pot
Aim: To have a better understanding of the expression and regulation of versican isoforms in neural precursor cells (NPC) and oligodendrogliogenesis. Methods:By
本试验以优质杂交油菜品种黔油14号的四个不同世代(P1,P2,F1和F2)为供试材料,对其九个主要农艺数量性状进行杂种优势测定和基因效应相关分析,并明确了各性状的最适遗传模型、基因效应和遗传率。1杂种优势黔油14号九个农艺性状在F1代均表现出一定的杂种优势,其中仅分枝高度小于1,株高和主花序长超过1.1,其余各性状均为1.0-1.1之间;在中亲优势上,仅分枝高度呈现负值,株高和主花序长超过0.1,
编辑同志:我们浙江省义乌市江东街道新兴村村委会的换届选举,藐视国法,存在着严重贿选作弊现象。我村选民总数有486人,贿选人数多达300人以上,贿选数额40余万元。其中村主任
Y2001-62771-593 0121802精神病医院死亡率分析的分类方法论=A categoriza-rion methodology for the analysis of the mortality rate inpsychiatric hospitals[会,英]/Ball
利用高丹草(314A×ZK)的F6:7重组自交系(RIL)群体200个株系,于2007年在内蒙古农业大学教学农场种植,对RIL群体进行了评价,并对株高、叶片数等8个重要农艺性状进行了方差分析、偏相关分析和多元逐步回归分析的研究;同时采用SSR分子标记技术对此RIL群体的株系进行标记,其主要结果如下:1. 8个性状在重组自交系群体中均存在双向超亲分离,分布频率大致接近正态分布。8个农艺性状在双亲之间
丹参(Salvia miltiorrhiza Bge.)作为一种传统中药,在临床上广泛用于治疗心脑血管疾病、慢性肾功能衰竭、神经衰弱性失眠、痛经及肝硬化。丹参化学成分复杂。研究表明,丹参中
Aim: To investigate whether the circadian expression of central clock genes and angiotensin Ⅱ type 1 (AT1) receptors was altered in sinoaortic-denervated (SAD)