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[目的 ]测定恶性疟原虫海南株 (FCC1/HN)环状体感染红细胞表面抗原 (RESA)基因 3′端部分基因序列 ,比较FCC1/HN与国外分离株RESA序列的差异。 [方法 ]应用PCR技术扩增RESA基因 3′端部分序列 ,将其克隆入pMD18 T载体。阳性重组克隆经酶切及PCR鉴定后 ,用双脱氧链末端终止法进行基因序列测定 ,并用分子生物学软件进行基因结构和同源性分析。 [结果 ]用PCR成功扩增出约 846bp的RESA基因特定片段 ,阳性克隆经酶切及PCR扩增确定。基因序列分析表明 ,我国恶性疟原虫FCC1/HN株与国外FC2 7,PaloAlto ,NF7株RESA基因序列有不同程度的差异。 [结论 ]确定了恶性疟原虫FCC1/HN株RESA基因 3′端序列。同源性分析表明 ,FCC1/HN株RESA序列与其他分离株存在一定差异
[Objective] To determine the partial sequence of 3’-end gene of erythrocyte surface antigen (RESA) gene of Plasmodium falciparum strain Hainan (FCC1 / HN) and compare the difference between FCC1 / HN and RESA sequence of foreign isolates. [Method] The 3 ’end sequence of RESA gene was amplified by PCR and cloned into pMD18 T vector. The positive recombinant clones were identified by restriction enzyme digestion and PCR. The gene sequences were determined by dideoxy chain termination method and the molecular structure and homology analysis were performed by molecular biology software. [Result] The specific fragment of about 846 bp RESA gene was amplified by PCR. The positive clones were confirmed by restriction enzyme digestion and PCR amplification. Gene sequence analysis showed that the sequence of RESA gene of P. falciparum FCC1 / HN strain in China was different from those in other foreign countries, such as FC27, PaloAlto and NF7 strains. [Conclusion] The 3 ’end sequence of RESA gene of Plasmodium falciparum FCC1 / HN strain was identified. Homology analysis showed that the RES1 sequence of FCC1 / HN strain was different from other isolates