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目的:研究成年大鼠心脏成纤维细胞(cardiac fibroblasts,CFs)在血管紧张素Ⅱ刺激下单核细胞趋化蛋白-1(monocyte chemotaxia protein-1,MCP-1)表达情况,探讨高血压合并心脏损害的可能机制。 方法:成年大鼠CFs体外培养,在不同浓度血管紧张素Ⅱ不同作用时间刺激下,应用免疫蛋白印迹法和ELISA法分别测定细胞中和培养上清中MCP-1的含量。 结果:①在细胞内和培养上清中均有MCP-1表达。②随着血管紧张素Ⅱ、刺激浓度的增加和刺激时间的延长,细胞内和上清中MCP-1的表达量也逐渐增加。在1×10~(-11),1×10~(-9),1×10~(-8),1×10~(-7),1×10~(-6)mol/L的血管紧张素Ⅱ作用下,上清中MCP-1的含量分别为(1.60±0.21),(3.18±0.15),(4.70±0.22),(9.18±0.52),(8.75±0.42)μg/L,与对照组(1.13±0.09)μg/L比较,除1×10~(-11)mol/L组外,差异均有显著性意义(t=26.21～39.67,P<均0.05)。③在1×10~(-7)mol/L的血管紧张素Ⅱ作用下,3,6,12和24h MCP-1的表达量分别为(2.13±0.31),(3.25±0.20),(5.28±0.50)和(9.18±0.52)μg/L,与空白对照组(1.13±0.09)μg/L比较差异均有显著性意义(t=6.93～34.11,P均<0.05)。 结论:成年大鼠CFs的MCP-1的表达对血管紧张素Ⅱ的刺激有浓度和时间依赖性。CFs的MCP-1的表达可能参与高血压时?
Objective: To investigate the expression of monocyte chemotaxia protein-1 (MCP-1) in adult rat cardiac fibroblasts (CFs) stimulated by angiotensin Ⅱ and to explore the relationship between hypertension and heart Possible mechanism of damage. Methods: Adult rat CFs were cultured in vitro and stimulated with different concentrations of angiotensin Ⅱ for a time. The contents of MCP-1 in the cells and in the culture supernatant were determined by immunoblotting and ELISA. Results: ① The expression of MCP-1 in both cells and culture supernatants. ② With the increase of angiotensin Ⅱ, stimulation concentration and stimulation time, the expression of MCP-1 in cells and supernatant also increased gradually. Blood vessels in 1 × 10 ~ (-11), 1 × 10 ~ (-9), 1 × 10 ~ (-8), 1 × 10 ~ (-7) and 1 × 10 ~ (-6) mol / The levels of MCP-1 in the supernatant were (1.60 ± 0.21), (3.18 ± 0.15), (4.70 ± 0.22), (9.18 ± 0.52) and (8.75 ± 0.42) μg / L, Compared with control group (1.13 ± 0.09) μg / L, the difference was significant except 1 × 10 ~ (-11) mol / L group (t = 26.21 ~ 39.67, P <0.05). ③ Under the action of 1 × 10 -7 mol / L angiotensin Ⅱ, the expression of MCP-1 at 3, 6, 12 and 24 h were (2.13 ± 0.31), (3.25 ± 0.20), (5.28 ± 0.50) and (9.18 ± 0.52) μg / L respectively, which was significantly different from that of the blank control group (1.13 ± 0.09) μg / L (t = 6.93 ~ 34.11, P <0.05). CONCLUSION: The expression of MCP-1 in CFs of adult rats has a concentration-dependent and time-dependent effect on the stimulation of angiotensin II. The expression of MCP-1 in CFs may be involved in hypertension?