Isolation and Purification of Xanthones from Lomatogonium carinthiacum (Wulf) Reichb by HSCCC and HP

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[Objective]To isolate and purify the xanthones from Lomatogonium carinthiacum. [Methods] High-speed counter-current chromatography method(HSCCC),combined with macroporous resin chromatography were applied to the separation and purification of xanthones from L. carinthiacum; and the chemical structural identification was carried out by spectroscopic methods,including HR-ESI-MS and extensive1 D and 2D NMR techniques. [Results]Seven xanthones were obtained.,namely 1,4,8-trimethoxyxanthone-6-O-β-D-glucoronyl-(1-6)-O-β-D-glucoside(1),1,2,6-trihydroxyxanthone-8-O-β-D-glucoside(2),1,5,8-trihydroxy-3-methoxyxanthone(3),1,8-dihydroxy-3,4,5-trimethoxyxanthone(4),8-hydroxy-1,3,5-trimethoxyxanthone(5),1-hydroxy-3,5,8-trimethoxyxanthone(6) and 1,8-dihydroxy-4,5-dimethoxy-6,7-methylenedioxyxanthone(7) [Conclusions]Compounds 1,2 and 7 were separated from L. carinthiacum for the first time. [Objective]To isolate and purify the xanthones from Lomatogonium carinthiacum. [Methods] High-speed counter-current chromatography method(HSCCC),combined with macroporous resin chromatography were applied to the separation and purification of xanthones from L. carinthiacum; and the chemical Structural investigation was carried out by spectroscopic methods, including HR-ESI-MS and extensive1 D and 2D NMR techniques. [Results]Seven xanthones were obtained.,namely 1,4,8-trimethoxyxanthone-6-O-β-D-glucoronyl -(1-6)-O-β-D-glucoside(1),1,2,6-trihydroxyxanthone-8-O-β-D-glucoside(2),1,5,8-trihydroxy-3-methoxyxanthone (3) 1,8-dihydroxy-3,4,5-trimethoxyxanthone(4),8-hydroxy-1,3,5-trimethoxyxanthone(5),1-hydroxy-3,5,8-trimethoxyxanthone(6) And 1,8-dihydroxy-4,5-dimethoxy-6,7-methylenedioxyxanthone(7) [Conclusions]Compounds 1,2 and 7 were separated from L. carinthiacum for the first time.
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