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目的通过观察依托咪酯后处理对大鼠脑缺血再灌注损伤(Ischemia-reperfusion,I/R)丙二醛(malondialdehyde,MDA)和超氧化物歧化酶(super oxide dismutase,SOD)的影响,探讨依托咪酯对大鼠脑缺血再灌注损伤的脑保护机制及依托咪酯脑保护的适宜剂量。方法成年健康Wistar大鼠40只,随机分为5组(n=8):假手术组(Sham组)、脑缺血再灌注组(I/R组)、依托咪酯后处理1,2,3组(Eto1,2,3组,分别给予不同剂量的依托咪酯)。除Sham组外,其他组夹闭双侧颈总动脉脑缺血造型,缺血20min后给予相应处理并恢复血流,24h后处死动物取脑组织测定其MDA含量以及SOD活性,并取脑组织视交叉平面在HE染色下观察其病理改变。结果与Sham组比较,I/R组MDA含量显著增高,SOD活性明显降低(P均<0.05);与I/R组比较,Eto1,2,3组可降低MDA含量,提高SOD活性(P均<0.05);Eto3组与Eto1、2组比较,MDA含量增高、SOD活性降低(P均<0.05)。结论依托咪酯后处理对大鼠脑缺血再灌注损伤有保护作用,其机制可能与抑制氧自由基有关,Eto1、2组的脑保护作用强于Eto3组。
Objective To observe the effects of etomidate postconditioning on malondialdehyde (MDA) and superoxide dismutase (SOD) in rats with cerebral ischemia-reperfusion (I / R) To investigate the protective mechanism of etomidate on cerebral ischemia-reperfusion injury in rats and the appropriate dose of etomidate brain protection. Methods Forty adult Wistar rats were randomly divided into five groups (n = 8): sham operation group (Sham group), cerebral ischemia reperfusion group (I / R group), etomidate postconditioning 1, 3 groups (Eto1,2,3 group, were given different doses of etomidate). Except for the Sham group, the other groups were occluded with bilateral common carotid arteries for cerebral ischemia. After ischemia for 20 min, the rats were treated and resumed their blood flow. After 24 h, the animals were sacrificed to determine their MDA content and SOD activity. Brain tissue The optic chiasm plane was observed under HE staining for pathological changes. Results Compared with Sham group, MDA content in I / R group was significantly increased and SOD activity was significantly decreased (P <0.05). Compared with I / R group, Eto1,2,3 group decreased MDA content and increased SOD activity <0.05). Compared with Eto1 and 2 groups, MDA content increased and SOD activity decreased in Eto3 group (all P <0.05). Conclusion Etomidate may have a protective effect on cerebral ischemia-reperfusion injury in rats. The mechanism may be related to the inhibition of oxygen free radicals. Eto1,2 group is more protective than Eto3 group.